Inhibition of Hepatitis B Virus Replication by Homoharringtonine, IFN-α1b, Hydroxycamptothecin and Norfloxacin

RAO Min; ZHANG Shu-ling; DONG Ji-hua; LI Shu-li

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June 2006

RAO Min, ZHANG Shu-ling, DONG Ji-hua and LI Shu-li. Inhibition of Hepatitis B Virus Replication by Homoharringtonine, IFN-α1b, Hydroxycamptothecin and Norfloxacin[J]. Virologica Sinica, 2006, 21(3): 284-287.

Citation: RAO Min, ZHANG Shu-ling, DONG Ji-hua, LI Shu-li. Inhibition of Hepatitis B Virus Replication by Homoharringtonine, IFN-α1b, Hydroxycamptothecin and Norfloxacin .VIROLOGICA SINICA, 2006, 21(3) : 284-287.

高三尖杉酯碱等四种药物的体外抑制乙肝病毒的实验研究

饶敏 ¹ ,
张淑玲 ^1*,, ,
董继华 ² ,
李淑莉 ¹

1.
1.华中科技大学同济医学院附属协和医院感染科，湖北武汉 430022
2.
华中科技大学同济医学院附属协和医院病毒研究室，湖北武汉 430022

通讯作者： 张淑玲, ;

摘要

探讨高三尖杉酯碱、干扰素α1b、羟基喜树碱和诺氟沙星这四种药物对乙肝病毒的体外抑制作用。利用HBV全基因组转染的人肝癌细胞系（HepG2.2.15），采用CPE方法观察四种药物对HepG2.2.15的毒性大小，在最大无毒浓度下，将药物加入HepG2.2.15培养8天收集培养液，用ELISA法和荧光定量PCR法分别测定药物对细胞培养上清中HBsAg和HBeAg及HBV-DNA的抑制作用。结果显示在体外高三尖杉酯碱和干扰素α1b对乙肝病毒均有明显的抑制作用，羟基喜树碱对乙肝病毒仅有一定的抑制作用，诺氟沙星则在各浓度均未见对乙肝病毒有抑制作用。本实验证实了高三尖杉酯碱在体外对乙肝病毒有明显的抑制作用，为临床抗乙肝药物的研究提供了一定的理论依据。
- 高三尖杉酯碱
- , HepG2.2.15
- , HBVDNA
- , 荧光定量PCR

Inhibition of Hepatitis B Virus Replication by Homoharringtonine, IFN-α1b, Hydroxycamptothecin and Norfloxacin

1.
1.Infectious Office，Union Hospital, Tong Ji Medical College of Huazhong University of Science and Technology，Wuhan 430022,China
2.
Virus Study Office, Union Hospital, Tong Ji Medical College of Huazhong University of Science and Technology，Wuhan 430022,China

Corresponding author: ZHANG Shu-ling,

Abstract

The inhibitory effects of homoharringtonine、IFN-α1b 、hydroxycamptothecin and norfloxacin on Hepatitis B virus（HBV）replication in vitro was investigated. Different concentrations of each drug were added to the culture medium of HepG2.2.15 cell line transfected with HBV. The Cytopathic effects（CPE）and cell toxicity of each drug was observed. Under the maximum non-toxic conentration(TC0)，ELISA and fluorescent quantified PCR were used for determining HBsAg、HBeAg and HBVDNA in the culture medium. The results showed that homoharringtonine and IFN-α1b had an apparent inhibitory effect to HBV while Hydroxycamptothecinhad had only a weak effect to HBV replication. Norfloxacin had no inhibition effect to HBV at any concentration. This data t provide part of the experimental evidence needed to initiate clinical trials to evaluate homoharringtonine as an anti-HBV drub.
- Homoharringtonine
- , HepG2.2.15
- , HBV DNA
- , Fluorescent quantified PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Inhibition of Hepatitis B Virus Replication by Homoharringtonine, IFN-α1b, Hydroxycamptothecin and Norfloxacin

Corresponding author: ZHANG Shu-ling,

1. 1.Infectious Office，Union Hospital, Tong Ji Medical College of Huazhong University of Science and Technology，Wuhan 430022,China
2. Virus Study Office, Union Hospital, Tong Ji Medical College of Huazhong University of Science and Technology，Wuhan 430022,China

Abstract: The inhibitory effects of homoharringtonine、IFN-α1b 、hydroxycamptothecin and norfloxacin on Hepatitis B virus（HBV）replication in vitro was investigated. Different concentrations of each drug were added to the culture medium of HepG2.2.15 cell line transfected with HBV. The Cytopathic effects（CPE）and cell toxicity of each drug was observed. Under the maximum non-toxic conentration(TC0)，ELISA and fluorescent quantified PCR were used for determining HBsAg、HBeAg and HBVDNA in the culture medium. The results showed that homoharringtonine and IFN-α1b had an apparent inhibitory effect to HBV while Hydroxycamptothecinhad had only a weak effect to HBV replication. Norfloxacin had no inhibition effect to HBV at any concentration. This data t provide part of the experimental evidence needed to initiate clinical trials to evaluate homoharringtonine as an anti-HBV drub.