Expression of FMDV P1+2A Gene in BHK-21 Cells

GONG Zhen-li; LIU Xiang-tao; TIAN Hong; WU Jin-yan; DAI Xing-guo; SHANG You-jun; CHEN Guo-dong

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October 2006

GONG Zhen-li, LIU Xiang-tao, TIAN Hong, WU Jin-yan, DAI Xing-guo, SHANG You-jun and CHEN Guo-dong. Expression of FMDV P1+2A Gene in BHK-21 Cells[J]. Virologica Sinica, 2006, 21(5): 454-457.

Citation: GONG Zhen-li, LIU Xiang-tao, TIAN Hong, WU Jin-yan, DAI Xing-guo, SHANG You-jun, CHEN Guo-dong. Expression of FMDV P1+2A Gene in BHK-21 Cells .VIROLOGICA SINICA, 2006, 21(5) : 454-457.

口蹄疫病毒P1＋2A基因在BHK－21细胞中的表达

1.
中国农业科学院兰州兽医研究所农业部畜禽病毒学重点实验室，甘肃兰州 730046

摘要

本研究将已构建好的、包含口蹄疫病毒P1+2A基因的重组表达质粒pQE-Tri/P1+2A经质脂体2000转染哺乳动物细胞BHK-21，转染后一定时间进行检测。通过SDS-PAGE、Western-blotting、荧光抗体染色、ELISA等检测方法表明，FMDV P1+2A基因片段在BHK-21细胞中成功表达，表达的蛋白能被口蹄疫阳性血清所识别而且具有良好的生物学活性。这一结果的取得，为进一步研制新型口蹄疫基因工疫苗及其配套诊断试剂奠定了基础。
- 口蹄疫病毒
- , P1+2A基因
- , 表达载体pQE-Trisystem
- , BHK-21细胞转染

Expression of FMDV P1+2A Gene in BHK-21 Cells

1.
Key Laboratory of Animal Virology, Ministry of Agriculture Lanzhou Veterinary Research Institute, Lanzhou 730046,China

Abstract

In this study, recombinant expression plasmid pQE-Tri/P1+2A was constructed and then transfected into BHK-21 cell line mediated by liposome 2000. The results of SDS-PAGE、Western-blotting、fluorescence antibody and ELISA showed that FMDV P1+2A protein was expressed in BHK-21 cell line successfully and can be recognized by FMD positive serum. The result will lay a foundation for study of FMDV gene engineered vaccines as well as the development of diagnostic reagents.
- Foot-and-mouth Disease Virus
- , P1+2A gene
- , Expression vector:pQE-Trisystem
- , BHK-21 Cells transfection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of FMDV P1+2A Gene in BHK-21 Cells

1. Key Laboratory of Animal Virology, Ministry of Agriculture Lanzhou Veterinary Research Institute, Lanzhou 730046,China

Abstract: In this study, recombinant expression plasmid pQE-Tri/P1+2A was constructed and then transfected into BHK-21 cell line mediated by liposome 2000. The results of SDS-PAGE、Western-blotting、fluorescence antibody and ELISA showed that FMDV P1+2A protein was expressed in BHK-21 cell line successfully and can be recognized by FMD positive serum. The result will lay a foundation for study of FMDV gene engineered vaccines as well as the development of diagnostic reagents.