Construction of coxsackievirus B5 viruses with luciferase reporters and their applications <i>in vitro</i> and <i>in vivo</i>

Shangrui Guo; Meng Xun; Tingting Fan; Xinyu Li; Haoyan Yao; Xiaozhen Li; Bo Wu; Hang Yang; Chaofeng Ma; Hongliang Wang

doi:10.1016/j.virs.2023.05.010

August 2023

Citation: Shangrui Guo, Meng Xun, Tingting Fan, Xinyu Li, Haoyan Yao, Xiaozhen Li, Bo Wu, Hang Yang, Chaofeng Ma, Hongliang Wang. Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo .VIROLOGICA SINICA, 2023, 38(4) : 549-558. http://dx.doi.org/10.1016/j.virs.2023.05.010

Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo

Shangrui Guo ^a ,
Meng Xun ^a ,
Tingting Fan ^a ,
Xinyu Li ^a ,
Haoyan Yao ^b ,
Xiaozhen Li ^a ,
Bo Wu ^a ,
Hang Yang ^a ,
Chaofeng Ma ^c ,
Hongliang Wang ^{a
,,}

a. Department of Pathogen Biology and Immunology, Xi’an Jiaotong University Health Science Center, Xi’an, 710061, China;
b. Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, 710061, China;
c. Department of Viral Diseases Laboratory, Xi’an Center for Disease Control and Prevention, Xi’an, 710061, China

Corresponding author: Hongliang Wang, hongliangwang@xjtu.edu.cn
Received Date: 01 February 2023
Accepted Date: 11 May 2023
Available online: 26 May 2023

Abstract

Coxsackievirus belongs to the Picornaviridae family and is one of the major pathogens that cause hand, foot and mouth disease (HFMD) in infants and children with potential serious complications and even deaths. The pathogenesis of this virus is not fully elucidated and no vaccine or antiviral drug has been approved. In this study, a full-length infectious cDNA clone of coxsackievirus B5 virus was assembled and the recombinant virus displayed similar growth kinetics and ability to cause cytopathic effects as the parental virus. Luciferase reporter was then incorporated to generate both full-length and subgenomic replicon (SGR) reporter viruses. The full-length reporter virus is suitable for high-throughput antiviral screening, while the SGR is a useful tool to study viral-host interactions. More importantly, the full-length reporter virus has also been shown to infect the suckling mouse model and the reporter gene could be detected using an in vivo imaging system, thus providing a powerful tool to track viruses in vivo. In summary, we have generated coxsackievirus B5 reporter viruses and provided unique tools for studying virus-host interactions in vitro and in vivo as well as for high-throughput screenings (HTS) to identify novel antivirals.
- Coxsackievirus
- , Luciferase
- , High-throughput screening
- , Mouse model

Electronic Supplementary Material

10.1016j.virs.2023.05.010-ESM.docx
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