STUDIES ON PURIFICATION, MORPHOLOGY AND DOUBLE STRANDED RNA OF RICE RAGGED STUNT VIRUS
Abstract: The partial Purified preparations of Rice Ragged Stunt Virus (RRSV) from fresh diseased rice leaves could be obtained in the high yield by the combination of the techniques of clarification with CCI_4, precipitation with Polyethylene Glycol,differential centrifugations and linear sucrose gradient centrifugations. The preparations showed the maxium of the ultra-violet absorption spectra was at 260 nm and the value of the A_(260)/A_(280) ratio was 1.6. By Electron microscopy the RRSV particles showed spiked i...