1986 Vol.01(3)

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Review

SCIENCE AND TECHNOLOGY

GAO Shang-Yin

1986, 1(3): 1

SOME ADVANCES OF VIRUSES APPLIED IN AGRICULTURE

LUO Ming-Dian

1986, 1(3): 3

PREPARATION AND PRELIMINARY APPLICATION OF CYTOMEGALOVIRUS ANTIGEN—GLASS SLIDE TO RAPID DIAGNOSIS

WANG Hui-Min, LUO Lin, GONG Zhen-Kui

1986, 1(3): 13

Cytomegalovirus (CMV) Antigen-glass slide was prepared with human enbryonic lung cells (HL) infected with CMV(AD169 strain). An indirect immunoperoxdase technique (IPA) carried out on the glass slides was preliminary applied to rapid diagnosis of CMV. Complement fixation test (CFT) and immunofluorescence antibody technique (IFA)were run in parallel. The results of detection CMV IgG antibody in 103 sera and CMV IgG and IgM antibody in 18 paired sera showed that IPA is specific, while results of IPA were coin...

ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MCAB TO JEV AND THEIR USES FOR ANTIGENIC ANALYSIS

SHU Yong-Xin, LI De-Fu, LI Xiu-Hua, WANG Jin-Feng, ZHANG Guo-Ming, GUO Wei

1986, 1(3): 19

Thriteen hybridoma cell lines secreting McAbs against IEV have been established. Six of them were selected for study. The characterization of the six cell lines of McAb indicated that three of them were JEV—species specific and the other three were JEV—subgroup specific. In this paper, 12 JE virus strains, including 10 Chinese strains, one Japanese strain and one attenuated virus, were selected for antigenic analysis with the six cell lines of McAb. The result showed that most of those Chinese strains were ...

THE INFLUENCE OF SMALL DOSAGES OF INTERFERON ON VIRUSES ON DIFFERENT STAGES OF CHRONIC HEPATITIS B

XIONG Kai-Jun, HUANG Hua-Fang, JIN Wei-E, YAO Meng-Lin, ZENG Lin-Lan, HE Yong-Wen, HE Sheng-Song, WU Zhang-Qi

1986, 1(3): 24

In this paper 97 HBsAG positive cases and 60 HBeAg positive cases of chronic hepatitis B were treated with small dosages of interferon for one to six months. The viral targets were HBsAg HBeAg, anti-HBe, DNAP, HBV-DNA, After one month of post-treatment, six targets had not improved conspicuously in 97 cases. After two months of post-treatment, DNAP and HBeAg positive rates declined conspicuously in only 77 cases of disease, 5 viral targets all showed a statistically signifi cant decline for HBV-DNA from 73....

THE AVAILABILITY OF MONOCLONAL ANTIBODY IN DETECTING HERPES SIMPLEX VIRUS ANTIGEN WITH ELISA

SHEN Qian, TANG Jia-Qi, QIN Ke-Feng, HONG Mei-Xian, JIANG Chao-Zhun

1986, 1(3): 31

A double sandwich ELISA has been developed to detect herpes simplex virus(HSV) antigen in 70 clinical specimens, A valuation on availability of Monoclonal Anti body (McAb) in rapidly detecting HSV antigen was set, The sensitivity of McAb ELISA was 20-30 TCID50/0.1ml. The concordance of detecting HSV between McAb ELISA and virus isolation in clinical specimens was 85%. The mixture of two McAbs was used, it might elevate the sensitivity of ELISA.

RESEARCHES ON THE HEMAGGLUTININ(HAN) OF HEMORRHAGIC FEVER WITH RENAL SYNDROME VIRUSES (HFRSV) Ⅰ. A PRELIMINARY STUDY ON THE HEMAGGLUTING ACTIVITY OF HFRSV

XIA Dong-Xiang, HONG Mei-Xian, JIANG Chao-Liang, MA Wen-Yu, NI Da-Dan, ZHOU Ning

1986, 1(3): 37

2-1 day old suckling mice were inoculated ic with 8 HFRSV strains (Chen, A_(216), A_9, H_(8205), C_4, R_(22), 76/118) respectively. The brain tissue of the mice was harvested immediately before death and prepared into HAN by sucrose-acetone method. The HA activity and HI reactivity were tested. It was demonstrated that the HA activity varied with HFRSV strains and was associated with their virulence (LD_(50)) to the suckling mice. There was no cross reaction between HFRSVHAN and other viral immune sera. The...

EFFECT OF ASTRAGALUS MEMBRANACEUS (AM) ON COXSACKIE B-2 VIRUS INFECTED RAT BEATING HEART CELLS IN CULTURE

YANG Ying-Zhen, GUO Ji, JIN Pei-Yang, PU Shou-Ru, Cheng-Juan-Ru, JIN Ru-Xian, CHEN Hao-Zhu, GONG Jie-Xun, CHEN Ju-Yang

1986, 1(3): 43

The protective effect of Astragalus Membranaceus(AM) on Coxsackie B-2 virus infected newborn rat heart cell cultures was observed. The cardiac enzymes-lactic acid dehydrogenase (LDH) and glutamic oxaloacetic transaminase (SGOT) in infected group were much higher than in those groups of uninfected. AM control, and AM treated 1 hr after inoculation with 100 TCID50 Coxsackie B-2 virus for 2—5 days (p<0.01). The beating percentage began to decrease in infected group at the 2nd day and-no beating was shown 3—...

SEROLOGICAL STUDIES ON THE NUCLEAR POLYHEDROSIS VIRUS OF BOARMIA OBLIQUA

ZHAO Huai-Yu, CHEN Li-Hua, ZHANG Li-Ren

1986, 1(3): 50

Serological relationships of the nuclear polyhedrosis virus of Boarmia Obliqua with other six kinds of insect baculoviruses, namely, those of Euproctis Pseudoconspersa, Buzura suppressria Prodenia litura, Bombyx mori Philosamia cynhtia ricini and Antheraea pernyi, were studied by common serological methods such as counter-immuno-electropho rests and immunodiffusion, and otherwiseby enzyme-linked immunosorbent assay (ELISA).Resuhs obtained in the present study indicated that the antiserum of the nuclear poly...

THE STRUCTURE POLYPEPTIDES FROM CLOSTERA ANASTOMOSIS GRANULOSIS VIRUS

MENG Xiao-Lin

1986, 1(3): 58

The structure polypetides of Clostera anastomosis granulosis virus were studied by vertical slab SDS-PAGE. The result shows that Cas GV granulin passed though Sepharose-6 Bconsists of on major polypetide Component with an estimated molecular weight of 32×10~3d. The virus particle revealed at Least compositon of 23 bands with molecular weight ranging from 13 to 85×10~3d. 3 major polypeptide of virion were positivly reacted by PAS staining detection and could have glycoproteins on the virion.

STUDIES ON PURIFICATION, MORPHOLOGY AND DOUBLE STRANDED RNA OF RICE RAGGED STUNT VIRUS

WU Jian-Hua, LIU Hui-Hua, CHEN Zuo-Yi, GONG Zu-Xun, LIN Rui-Fen, JIN Deng-Di

1986, 1(3): 69

The partial Purified preparations of Rice Ragged Stunt Virus (RRSV) from fresh diseased rice leaves could be obtained in the high yield by the combination of the techniques of clarification with CCI_4, precipitation with Polyethylene Glycol,differential centrifugations and linear sucrose gradient centrifugations. The preparations showed the maxium of the ultra-violet absorption spectra was at 260 nm and the value of the A_(260)/A_(280) ratio was 1.6. By Electron microscopy the RRSV particles showed spiked i...

A STUDY ON THE HEMOCYTES OF PHILOSAMIA CYNTHIA RICINI IN VITRO AND INFECTIVE EXPERIMENT WITH VIRUS

XIE Tian-En, Wang Lu-Ming, Liang Bu-Feng

1986, 1(3): 76

SILVER STAINING FOR DETECTION OF NANOGRAM AMOUNTS OF PROTEINS AND NUCLEIC ACIDS IN GELS

LIN Qi-Feng

1986, 1(3): 80

ISOLATION PURIFICATION AND BIOLOGICAL PROPERTY OF LEUKEMIA VIRUS FROM L_(6565) AND SRS TUMOR STRAINS

CHENG Li, ZHENG Bao-Fen

1986, 1(3): 85