Molecular epidemiology demonstrates that imported and local strains circulated during the 2014 dengue outbreak in Guangzhou, China

Geng Li; Pan Pan; Qiuyan He; Xiujuan Kong; Kailang Wu; Wei Zhang; Yuntao Liu; Huiting Huang; Jianbo Liu; Zhongde Zhang; De Wu; Xiaoping Lai; Xiaohong Liu; Jianguo Wu

doi:10.1007/s12250-016-3872-8

February 2017

Geng Li, Pan Pan, Qiuyan He, Xiujuan Kong, Kailang Wu, Wei Zhang, Yuntao Liu, Huiting Huang, Jianbo Liu, Zhongde Zhang, De Wu, Xiaoping Lai, Xiaohong Liu and Jianguo Wu. Molecular epidemiology demonstrates that imported and local strains circulated during the 2014 dengue outbreak in Guangzhou, China[J]. Virologica Sinica, 2017, 32(1): 63-72. doi: 10.1007/s12250-016-3872-8

Citation: Geng Li, Pan Pan, Qiuyan He, Xiujuan Kong, Kailang Wu, Wei Zhang, Yuntao Liu, Huiting Huang, Jianbo Liu, Zhongde Zhang, De Wu, Xiaoping Lai, Xiaohong Liu, Jianguo Wu. Molecular epidemiology demonstrates that imported and local strains circulated during the 2014 dengue outbreak in Guangzhou, China .VIROLOGICA SINICA, 2017, 32(1) : 63-72. http://dx.doi.org/10.1007/s12250-016-3872-8

分子流行病学研究结果显示输入株和本地株在2014年广州登革大暴发时共流行

李耿 ^1,2 ,
潘攀 ² ,
孔秀娟 ¹ ,
邬开朗 ¹ ,
张威 ² ,
刘云涛 ^1,2 ,
黄慧婷 ⁴ ,
刘建博 ³ ,
张仲德 ³ ,
吴德 ³ ,
赖小平 ⁴ ,
刘小虹 ⁵ ,
吴建国 ¹ ,

1.
广州中医药大学中药学院，广州，510006，中国
2.
病毒学国家重点实验室，武汉大学生命科学学院，武汉，430072，中国
3.
广州中医药大学第一附属医院，广州，510120，中国
4.
广东省中医院，广州，510120，中国
5.
广东省疾病预防控制中心，广州，511430，中国

收稿日期： 2016-09-18
录用日期： 2016-11-15
出版日期： 2017-01-23

摘要

登革热是一种全球范围内流行的疾病，2014年在广州暴发的登革疫情引起了约40,000例感染和5例死亡。为了探讨2014年广州地区登革病毒传播来源及其流行特征，我们收集了2014广州大暴发时期登革患者的血清样本，从168份样本中分离17株病毒并对其E基因进行测序，利用MEGA和BEAST软件构建其系统发育树，分析2014年广州登革毒株的可能传播来源。实验结果显示17株病毒株均为血清I型，其中8株为基因I型，9株为基因V型。2株基因I型毒株是由2014年马来半岛地区传入，9株基因V型毒株则是由2014年马来西亚和印度地区传入。此外，6株基因I型毒株推测是来自于广州可能已经形成的本地传播毒株，通过系统发生地理学的研究表明，本地传播的毒株由2009年泰国传入，在2013到2015年开始流行，并在2013年传至新加坡。综上所述，2014年不同血清型和基因型的登革病毒在广州共同流行，引起了登革疫情的大暴发。广州不仅是登革病毒的输入地，而且还可能已经成为了登革病毒的自然疫源地甚至成为其输出地区。
- 登革病毒
- , 系统进化分析
- , E基因
- , 本地传播

Molecular epidemiology demonstrates that imported and local strains circulated during the 2014 dengue outbreak in Guangzhou, China

Geng Li ^1,2 ,
Pan Pan ² ,
Qiuyan He ¹ ,
Xiujuan Kong ¹ ,
Kailang Wu ² ,
Wei Zhang ^1,2 ,
Yuntao Liu ⁴ ,
Huiting Huang ³ ,
Jianbo Liu ³ ,
Zhongde Zhang ³ ,
De Wu ⁴ ,
Xiaoping Lai ⁵ ,
Xiaohong Liu ¹ ,
Jianguo Wu ^1,3,,

1.
School of Chinese Meterla Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2.
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
3.
The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China
4.
Guangdong Province Traditional Chinese Medical Hospital, Guangzhou 510120, China
5.
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China

Corresponding author: Jianguo Wu, jwu@whu.edu.cn
ORCID: 0000-0002-8326-2895
Received Date: 18 September 2016
Accepted Date: 15 November 2016
Published Date: 23 January 2017

Abstract

The dengue virus (DENV) is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation aimed at identifying the transmission sources in this dengue epidemic. To analyze the phylogenetics of the 2014 dengue strains, the envelope (E) gene sequences from 17 viral strains isolated from 168 dengue patient serum samples were sequenced and a phylogenetic tree was reconstructed. All 17 strains were serotype I strains, including 8 genotype I and 9 genotype V strains. Additionally, 6 genotype I strains that were probably introduced to China from Thailand before 2009 were widely transmitted in the 2013 and 2014 epidemics, and they continued to circulate until 2015, with one affinis strain being found in Singapore. The other 2 genotype I strains were introduced from the Malaya Peninsula in 2014. The transmission source of the 9 genotype V strains was from Malaysia in 2014. DENVs of different serotypes and genotypes co-circulated in the 2014 dengue outbreak in Guangzhou. Moreover, not only had DENV been imported to Guangzhou, but it had also been gradually exported, as the viruses exhibited an enzootic transmission cycle in Guangzhou.
- dengue virus (DENV)
- , phylogenetic analysis
- , envelope (E) gene
- , enzootic transmission cycle

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