A Novel Vibriophage vB_VcaS_HC Containing Lysogeny-Related Gene Has Strong Lytic Ability against Pathogenic Bacteria

Chengcheng Li; Zengmeng Wang; Jiulong Zhao; Long Wang; Guosi Xie; Jie Huang; Yongyu Zhang

doi:10.1007/s12250-020-00271-w

April 2021

Chengcheng Li, Zengmeng Wang, Jiulong Zhao, Long Wang, Guosi Xie, Jie Huang and Yongyu Zhang. A Novel Vibriophage vB_VcaS_HC Containing Lysogeny-Related Gene Has Strong Lytic Ability against Pathogenic Bacteria[J]. Virologica Sinica, 2021, 36(2): 281-290. doi: 10.1007/s12250-020-00271-w

Citation: Chengcheng Li, Zengmeng Wang, Jiulong Zhao, Long Wang, Guosi Xie, Jie Huang, Yongyu Zhang. A Novel Vibriophage vB_VcaS_HC Containing Lysogeny-Related Gene Has Strong Lytic Ability against Pathogenic Bacteria .VIROLOGICA SINICA, 2021, 36(2) : 281-290. http://dx.doi.org/10.1007/s12250-020-00271-w

一株含溶源途径相关基因的新型强裂解性弧菌噬菌体vB_VcaS_HC

李成成 ^1,2 ,
王增猛 ^1,2 ,
赵久龙 ^1,2 ,
王龙 ¹ ,
谢国驷 ³ ,
黄倢 ³ ,
张永雨 ^1,2,,

1.
中国科学院青岛生物能源与过程研究所，山东省能源遗传学重点实验室，生物燃料重点实验室
2.
中国科学院大学
3.
中国水产科学院黄海水产研究所

通讯作者： 张永雨, zhangyy@qibebt.ac.cn, ORCID: http://orcid.org/0000-0002-5065-1237
收稿日期： 2020-03-02
录用日期： 2021-06-08
出版日期： 2020-08-07

摘要

为避免抗生素滥用带来的负面效应，在水产养殖业利用噬菌体感染来清除病原菌成为一种很有前景的养殖动物疾病防控途径。本研究我们以一株对虾致病菌Vibrio campbellii 18为宿主分离了1株烈性噬菌体，将其命名为vB_VcaS_HC。该噬菌体具一个正多边形头部和非伸缩性尾巴。其感染潜伏期为1.5h，感染病菌时单个细胞的噬菌体裂解量约为172 PFU/cell，5.5h内导致病菌的死亡率即高达96 %。该噬菌体具有一个81,566 bp的双链环形DNA基因组，其基因组内包含121个开放阅读框（ORF），然而71%的ORF都被注释为未知功能的蛋白。基于比较基因组和进化树分析， vB_VcaS_HC被归类到Caudovirales目，Siphoviridae科，Delepquintavirus属。在其基因组中，除了与噬菌体自身结构组装和DNA代谢相关的基因外，还包含了10个辅助代谢功能基因。其中丙酮酸磷酸二激酶（PPDK）基因是首次在噬菌体基因组中被发现，其编码的PPDK是糖酵解途径（EMP）的关键酶。有意思的是，该噬菌体基因组中亦存在一个溶源途径相关基因，即重组酶RecA基因，然而本研究中并没有发现该噬菌体能够转换为溶源状态。此外，在vB_VcaS_HC基因组中未发现任何细菌毒力基因和耐药性基因。该研究是首次对一株坎氏弧菌的烈性噬菌体进行了全面鉴定，并表明该噬菌体在坎氏弧菌感染性疾病防控方面有重要的应用潜力。
- 坎氏弧菌
- , 噬菌体
- , 溶源性
- , RecA
- , 辅助代谢基因

A Novel Vibriophage vB_VcaS_HC Containing Lysogeny-Related Gene Has Strong Lytic Ability against Pathogenic Bacteria

Chengcheng Li ^1,2 ,
Zengmeng Wang ^1,2 ,
Jiulong Zhao ^1,2 ,
Long Wang ¹ ,
Guosi Xie ³ ,
Jie Huang ³ ,
Yongyu Zhang ^1,2,,

1.
Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China
3.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

Corresponding author: Yongyu Zhang, zhangyy@qibebt.ac.cn
ORCID: http://orcid.org/0000-0002-5065-1237
Received Date: 02 March 2020
Accepted Date: 08 June 2021
Published Date: 07 August 2020

Abstract

To avoid the negative effects of antibiotics, using phage to prevent animal disease becomes a promising method in aquaculture. Here, a lytic phage provisionally named vB_VcaS_HC that can infect the pathogen (i.e., Vibrio campbellii 18) of prawn was isolated. The phage has an isometric head and a non-contractile tail. During phage infection, the induced host mortality in 5.5 h reached ca. 96%, with a latent period of 1.5 h and a burst size of 172 PFU/cell. It has an 81, 566 bp circular dsDNA genome containing 121 open reading frames (ORFs), and ca. 71% of the ORFs are functionally unknown. Comparative genomic and phylogenetic analysis revealed that it is a novel phage belonging to Delepquintavirus, Siphoviridae, Caudovirales. In the phage genome, besides the ordinary genes related to structure assembly and DNA metabolism, there are 10 auxiliary metabolic genes. For the first time, the pyruvate phosphate dikinase (PPDK) gene was found in phages whose product is a key rate-limiting enzyme involving Embden-Meyerhof-Parnas (EMP) reaction. Interestingly, although the phage has a strong bactericidal activity and contains a potential lysogeny related gene, i.e., the recombinase (RecA) gene, we did not find the phage turned into a lysogenic state. Meanwhile, the phage genome does not contain any bacterial virulence gene or antimicrobial resistance gene. This study represents the first comprehensive characterization of a lytic V. campbellii phage and indicates that it is a promising candidate for the treatment of V. campbellii infections.
- Vibrio campbellii
- , Phage
- , Lysogeny
- , RecA
- , Auxiliary metabolic gene

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