New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

Mengyuan Chen; Shuizhen He; Hualong Xiong; Dongxu Zhang; Shaojuan Wang; Wangheng Hou; Xiaomei Zhu; Jin Wang; Yang Huang; Congming Hong; Yubin Wu; Ruoyao Qi; Tianying Zhang; Quan Yuan; Tingdong Li; Yixin Chen; Shiyin Zhang; Shengxiang Ge; Jun Zhang; Ningshao Xia

doi:10.1016/j.virs.2022.07.001

October 2022

. doi: 10.1016/j.virs.2022.07.001

Citation: Mengyuan Chen, Shuizhen He, Hualong Xiong, Dongxu Zhang, Shaojuan Wang, Wangheng Hou, Xiaomei Zhu, Jin Wang, Yang Huang, Congming Hong, Yubin Wu, Ruoyao Qi, Tianying Zhang, Quan Yuan, Tingdong Li, Yixin Chen, Shiyin Zhang, Shengxiang Ge, Jun Zhang, Ningshao Xia. New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq .VIROLOGICA SINICA, 2022, 37(5) : 758-761. http://dx.doi.org/10.1016/j.virs.2022.07.001

基于PhIP-Seq技术新筛选出SARS-CoV-2 spike蛋白S2亚基的高亲和力结合短肽

陈梦媛 ^a ,
何水珍 ^b ,
熊华龙 ^c ,
张东旭 ^a ,
王邵娟 ^a ,
侯汪衡 ^a ,
朱晓妹 ^a ,
王进 ^c ,
黄洋 ^c ,
洪聪明 ^a ,
吴钰彬 ^c ,
戚若垚 ^c ,
张天英 ^a ,
袁权 ^a ,
李廷栋 ^a ,
陈毅歆 ^a ,
张师音 ^a,, ,
葛胜祥 ^a,, ,
张军 ^a ,
夏宁邵 ^a

a 厦门大学公共卫生学院, 国家传染病诊断试剂与疫苗工程技术研究中心, 分子疫苗学和分子诊断学国家重点实验室;
b 厦门市海沧医院;
c 厦门大学生命科学学院

通讯作者： 张师音, zhangshiyin@xmu.edu.cn; 葛胜祥, sxge@xmu.edu.cn;
收稿日期： 2021-07-28
录用日期： 2022-06-30

摘要

SARS-CoV-2感染引起的COVID-19带来了全球性的威胁，靶向SARS-CoV-2 S蛋白的预防与治疗将是控制COVID-19的有效手段。而高亲和力的结合分子是靶向研究的重要基础。本研究利用PhIP-Seq技术筛选出了靶向SARS-CoV-2 S蛋白的结合多肽Spep-1。经鉴定，该肽靶向结合S蛋白的S2亚基，其与S蛋白具有高亲和力和高特异性，Spep-1/S三聚体复合物的Kd值为1.7 nmol/L，Spep-1/S单体复合物的Kd值为2.14 nmol/L。本研究初步将其应用于血清中S蛋白的检测，基于Spep-1建立的ELISA方法，可以检测出血清中低至飞摩尔级别的S蛋白。本研究新鉴定出的结合多肽Spep-1，在未来的研究中经过理性修饰，将促进病毒入胞、体内病毒示踪、药物靶向递送等研究。
- 新型冠状病毒
- , S2亚基
- , 结合多肽
- , 噬菌体免疫沉淀-测序技术

New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

a State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, South Xiang'an Road, Xiamen, 361102, China;
b Xiamen Haicang Hospital, Haiyu Road, Xiamen, 361026, China;
c School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen, 361102, China

Corresponding author: Shiyin Zhang, zhangshiyin@xmu.edu.cn Shengxiang Ge, sxge@xmu.edu.cn
Received Date: 28 July 2021
Accepted Date: 30 June 2022

Abstract

Highlights
1 A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq.
2 Spep-1 showed nanomolar affinity and high specificity to spike protein.
3 S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples.
4 Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery.

References
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

Corresponding author: Shiyin Zhang, zhangshiyin@xmu.edu.cn

Corresponding author: Shengxiang Ge, sxge@xmu.edu.cn

a State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, South Xiang'an Road, Xiamen, 361102, China;

b Xiamen Haicang Hospital, Haiyu Road, Xiamen, 361026, China;

c School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen, 361102, China

Received Date: 28 July 2021

Accepted Date: 30 June 2022

Abstract: Highlights
1 A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq.
2 Spep-1 showed nanomolar affinity and high specificity to spike protein.
3 S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples.
4 Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery.

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基于PhIP-Seq技术新筛选出SARS-CoV-2 spike蛋白S2亚基的高亲和力结合短肽

摘要

New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

Abstract

References

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Electronic Supplementary Material

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通讯作者: 陈斌, bchen63@163.com