Temperature-regulated type II grass carp reovirus establishes latent infection in <i>Ctenopharyngodon idella</i> brain

Rui Jiang; Jie Zhang; Zhiwei Liao; Wentao Zhu; Hang Su; Yongan Zhang; Jianguo Su

doi:10.1016/j.virs.2023.04.006

June 2023

. doi: 10.1016/j.virs.2023.04.006

Citation: Rui Jiang, Jie Zhang, Zhiwei Liao, Wentao Zhu, Hang Su, Yongan Zhang, Jianguo Su. Temperature-regulated type II grass carp reovirus establishes latent infection in Ctenopharyngodon idella brain .VIROLOGICA SINICA, 2023, 38(3) : 440-447. http://dx.doi.org/10.1016/j.virs.2023.04.006

II型草鱼呼肠孤病毒受温度调控在草鱼脑中建立潜伏感染

蒋锐 ^a,b ,
张杰 ^a ,
廖志伟 ^a ,
朱文涛 ^a ,
苏航 ^a ,
张永安 ^a ,
苏建国 ^a,b,,

华中农业大学水产学院

通讯作者： 苏建国, sujianguo@mail.hzau.edu.cn
收稿日期： 2022-10-28
录用日期： 2023-04-26

摘要

草鱼呼肠孤病毒(GCRV)可引起草鱼和青鱼幼鱼广泛感染和死亡，具有高度季节性流行。以往的研究表明GCRV可在原发性感染后潜伏。在本研究中，我们探究了有GCRV感染或暴露史的无症状草鱼的II型GCRV (GCRV-II)的潜伏感染。我们发现，在潜伏感染期间，GCRV-II仅在草鱼的大脑中检测到，而不同于自然感染时观察到的多组织分布。GCRV-II在潜伏感染时只对大脑造成损伤；而在自然感染时，脑、心、眼组织的病毒载量相对较高。我们还在受感染的草鱼大脑中发现了病毒包涵体。此外，GCRV-II在草鱼体内的分布明显受环境温度的影响：病毒在低温时仅针对大脑，在高温时针对多种组织。本研究为了解GCRV-II潜伏感染和再激活的机制提供了思路，有助于预防和控制GCRV的大流行。
- 草鱼
- , 草鱼呼肠孤病毒
- , 潜伏感染
- , 脑

Temperature-regulated type II grass carp reovirus establishes latent infection in Ctenopharyngodon idella brain

Rui Jiang ^a,b ,
Jie Zhang ^a ,
Zhiwei Liao ^a ,
Wentao Zhu ^a ,
Hang Su ^a ,
Yongan Zhang ^a ,
Jianguo Su ^a,b,,

a. College of Fisheries, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China;
b. Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, 266237, China

Corresponding author: Jianguo Su, sujianguo@mail.hzau.edu.cn
Received Date: 28 October 2022
Accepted Date: 26 April 2023

Abstract

Grass carp reovirus (GCRV) causes extensive infection and death in grass carp and black carp fingerlings, with a highly seasonal prevalence. Previous studies suggested that GCRV can become latent after primary infection. In this study, we investigated type II GCRV (GCRV-II) latency in asymptomatic grass carp with GCRV infection or exposure history. We found that during latent infection, GCRV-II was detectable only in the brain of grass carp, unlike the multi-tissue distribution observed in natural infection. GCRV-II only caused damage to the brain during latent infection, while in natural infection, brain, heart, and eye tissues had relatively higher viral loads. We also discovered viral inclusion bodies in infected fish brains. Additionally, GCRV-II distribution in grass carp was notably affected by ambient temperature, with the virus targeting the brain only during low temperatures and multi-tissue distribution during high temperatures. This study provides insights into the mechanisms of GCRV-II latent infection and reactivation and contributes to the prevention and control of GCRV pandemics.
- Grass carp
- , Grass carp reovirus (GCRV)
- , Latent infection
- , Brain

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