A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II

Pei Huang; Zanheng Huang; Meihui Liu; Yujie Bai; Hongli Jin; Jingbo Huang; Xingqi Liu; Zhenhong Guan; Ming Duan; Haili Zhang; Yuanyuan Li; Sandra Chiu; Hualei Wang

doi:10.1016/j.virs.2023.05.012

August 2023

. doi: 10.1016/j.virs.2023.05.012

Citation: Pei Huang, Zanheng Huang, Meihui Liu, Yujie Bai, Hongli Jin, Jingbo Huang, Xingqi Liu, Zhenhong Guan, Ming Duan, Haili Zhang, Yuanyuan Li, Sandra Chiu, Hualei Wang. A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II .VIROLOGICA SINICA, 2023, 38(4) : 635-638. http://dx.doi.org/10.1016/j.virs.2023.05.012

猴痘病毒分支I、II的核酸可视化鉴别检测方法

黄培 ^a ,
黄赞恒 ^a ,
刘美慧 ^a ,
白玉洁 ^a ,
金宏丽 ^a ,
黄静波 ^a ,
刘星岐 ^a ,
关振宏 ^a ,
段铭 ^a ,
张海丽 ^a ,
李媛媛 ^a ,
Sandra Chiu ^b,, ,
王化磊 ^a,,

吉林大学动物医学学院

通讯作者： Sandra Chiu, qiux@ustc.edu.cn; 王化磊, wanghualei@jlu.edu.cn
收稿日期： 2023-02-07
录用日期： 2023-05-31

摘要

目前为止，MPXV已经传播到全世界103个国家和地区。2022年7月23日，世界卫生组织宣布猴痘（Mpox）疫情成为国际关注的公共卫生紧急情况。随着世界范围内感染病例的不断增加，开发一种MPXV的快速检测工具以提高非流行国家和地区的监测和检测能力具有重要意义。MPXV包括两个分支：分支I和分支II，两个支系具有不同的致病性。在本研究中，我们将等温扩增技术与免疫层析技术相结合，建立了一种MPXV核酸可视化检测方法，可在25分钟内快速鉴别分支I和分支II，其最低检测限为1拷贝/μL重组质粒。此外，该方法与引起相似临床症状的痘苗病毒、单纯疱疹病毒2型和水痘-带状疱疹病毒无交叉反应。总之，建立的MPXV分支I和II核酸可视化鉴别检测方法具有快速、简便、高敏感性、可分型等优点，有望用于Mpox疑似阳性样品的现地筛选。
- 猴痘病毒
- , 分支I和II
- , 鉴别
- , 快速核酸可视化检测

A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II

a. State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, 130062, China;
b. Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 244199, China

Corresponding author: Sandra Chiu, qiux@ustc.edu.cn Hualei Wang, wanghualei@jlu.edu.cn
Received Date: 07 February 2023
Accepted Date: 31 May 2023

Abstract

Highlights
1. The newly developed visual assay panel is a rapid and reliable tool to differentiate the clade I and clade II MPXV within 25 min.
2. The panel combines recombinase-aid amplification and immunochromatography, and detects as low as 1 copy/μL recombinant plasmid.
3. Visual assay panel shows no cross-reactivity with orthopoxviruses and herpesvirus that infect humans, such as vaccinia virus.

References
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3. Durski, K.N., McCollum, A.M., Nakazawa, Y., Petersen, B.W., Reynolds, M.G., Briand, S., Djingarey, M.H., Olson, V., Damon, I.K. Khalakdina, A., 2018. Emergence of Monkeypox-West and Central Africa, 1970-2017. MMWR Morb Mortal Wkly Rep. 67, 306-310.
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10. Peng, Q., Xie, Y., Kuai, L., Wang, H., Qi, J., Gao, G.F. Shi, Y., 2023. Structure of monkeypox virus DNA polymerase holoenzyme. Science. 379, 100-105.
11. WHO, 2022. Monkeypox:experts give virus variants new names. https://www.who.int/news/item/12-08-2022-monkeypox-experts-give-virus-variants-new-names, access:2023.5.31
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II

Corresponding author: Sandra Chiu, qiux@ustc.edu.cn

Corresponding author: Hualei Wang, wanghualei@jlu.edu.cn

a. State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, 130062, China;

b. Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 244199, China

Received Date: 07 February 2023

Accepted Date: 31 May 2023

Abstract: Highlights
1. The newly developed visual assay panel is a rapid and reliable tool to differentiate the clade I and clade II MPXV within 25 min.
2. The panel combines recombinase-aid amplification and immunochromatography, and detects as low as 1 copy/μL recombinant plasmid.
3. Visual assay panel shows no cross-reactivity with orthopoxviruses and herpesvirus that infect humans, such as vaccinia virus.

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猴痘病毒分支I、II的核酸可视化鉴别检测方法

摘要

A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II

Abstract

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Electronic Supplementary Material

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通讯作者: 陈斌, bchen63@163.com