. doi: 10.1016/j.virs.2023.06.001
Citation: Yalan Liu, Zhiyuan Zhu, Yuncheng Li, Mengshi Yang, Qinxue Hu. Migrasomes released by HSV-2-infected cells serve as a conveyance for virus spread .VIROLOGICA SINICA, 2023, 38(4) : 643-645.  http://dx.doi.org/10.1016/j.virs.2023.06.001

HSV-2感染细胞释放的迁移体介导病毒的传播

  • HSV-2的感染会增加HIV-1性传播风险。目前没有HSV-2的有效疫苗,也无证据表明其利用抗原变异逃逸宿主监控。HSV-2是否利用了某些目前未知的方式来介导病毒细胞间传播或者其他未知的免疫逃逸策略促进传播尚有待研究。迁移体在病毒传播过程中的作用及机制尚未有报道。本研究发现被HSV-2感染的细胞产生的迁移体中存在病毒颗粒,HSV-2感染小鼠组织中也存在包含病毒颗粒的迁移体,进一步研究还发现迁移体中的病毒能被转移到其他细胞并在其中复制。研究结果表明HSV-2可以利用“细胞—迁移体—细胞”的模式实现更广泛的传播,是对经典的HSV-2传播模式的重要补充,为发展新型靶向干预策略提供理论依据,而且建立的迁移体—病毒传播模型对其他病毒的研究具有重要参考意义。

Migrasomes released by HSV-2-infected cells serve as a conveyance for virus spread

  • Highlights
    1. Cells infected with HSV-2 release migrasomes containing HSV-2 virions.
    2. HSV-2 in the isolated migrasomes can be transmitted to uninfected cells and cause productive infection.
    3.It is the first time that migrasomes have been found to play a role in virus spread.

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    1. Baeten, J.M., McClelland, R.S., Corey, L., Overbaugh, J., Lavreys, L., Richardson, B.A., Wald, A., Mandaliya, K., Bwayo, J.J., Kreiss, J.K., 2004. Vitamin A supplementation and genital shedding of herpes simplex virus among HIV-1-infected women:a randomized clinical trial. J Infect Dis 189, 1466-1471.

    2. Bassani, S., Cingolani, L.A., 2012. Tetraspanins:Interactions and interplay with integrins. Int J Biochem Cell Biol 44, 703-708.

    3. Freeman, E.E., Weiss, H.A., Glynn, J.R., Cross, P.L., Whitworth, J.A., Hayes, R.J., 2006. Herpes simplex virus 2 infection increases HIV acquisition in men and women:systematic review and meta-analysis of longitudinal studies. AIDS 20, 73-83.

    4. Huang, Y., Zucker, B., Zhang, S., Elias, S., Zhu, Y., Chen, H., Ding, T., Li, Y., Sun, Y., Lou, J., Kozlov, M.M., Yu, L., 2019. Migrasome formation is mediated by assembly of micron-scale tetraspanin macrodomains. Nat Cell Biol 21, 991-1002.

    5. Jiang, D., Jiang, Z., Lu, D., Wang, X., Liang, H., Zhang, J., Meng, Y., Li, Y., Wu, D., Huang, Y., Chen, Y., Deng, H., Wu, Q., Xiong, J., Meng, A., Yu, L., 2019. Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation. Nat Cell Biol 21, 966-977.

    6. Jiao, H., Jiang, D., Hu, X., Du, W., Ji, L., Yang, Y., Li, X., Sho, T., Wang, X., Li, Y., Wu, Y.T., Wei, Y.H., Hu, X., Yu, L., 2021. Mitocytosis, a migrasome-mediated mitochondrial quality-control process. Cell 184, 2896-2910 e2813.

    7. Kimberlin, D.W., Whitley, R.J., 2007. Antiviral therapy of HSV-1 and -2, in:Arvin, A., Campadelli-Fiume, G., Mocarski, E., Moore, P.S., Roizman, B., Whitley, R., Yamanishi, K. (Eds.), Human Herpesviruses:Biology, Therapy, and Immunoprophylaxis, Cambridge.

    8. Liu, Y., Li, S., Rong, W., Zeng, C., Zhu, X., Chen, Q., Li, L., Liu, Z.H., Zen, K., 2020. Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury. Kidney Dis (Basel) 6, 422-433.

    9. Ma, L., Li, Y., Peng, J., Wu, D., Zhao, X., Cui, Y., Chen, L., Yan, X., Du, Y., Yu, L., 2015. Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Cell Res 25, 24-38.

    10. Sattentau, Q., 2008. Avoiding the void:cell-to-cell spread of human viruses. Nat Rev Microbiol 6, 815-826.

    11. Zhang, C., Li, T., Yin, S., Gao, M., He, H., Li, Y., Jiang, D., Shi, M., Wang, J., Yu, L., 2022a. Monocytes deposit migrasomes to promote embryonic angiogenesis. Nat Cell Biol 24, 1726-1738.

    12. Zhang, N., Gao, S., Zhang, L., 2022b. Chikungunya virus nsP1 induces migrasome formation. J Infect 85, e158-e161.

    13. Zhang, Y., Wang, J., Ding, Y., Zhang, J., Xu, Y., Xu, J., Zheng, S., Yang, H., 2020. Migrasome and Tetraspanins in Vascular Homeostasis:Concept, Present, and Future. Front Cell Dev Biol 8, 438.

    14. Zhao, X., Lei, Y., Zheng, J., Peng, J., Li, Y., Yu, L., Chen, Y., 2019. Identification of markers for migrasome detection. Cell Discov 5, 27.

    15. Zhu, M., Zou, Q., Huang, R., Li, Y., Xing, X., Fang, J., Ma, L., Li, L., Yang, X., Yu, L., 2021. Lateral transfer of mRNA and protein by migrasomes modifies the recipient cells. Cell Res 31, 237-240.

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    Migrasomes released by HSV-2-infected cells serve as a conveyance for virus spread

      Corresponding author: Yalan Liu, liuyl@wh.iov.cn
      Corresponding author: Qinxue Hu, qhu@wh.iov.cn
    • a. State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China;
    • b. Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China;
    • c. Institute for Infection and Immunity, St George's University of London, London, SW17 0RE, UK;
    • d. Hubei Jiangxia Laboratory, Wuhan, 430200, China

    Abstract: Highlights
    1. Cells infected with HSV-2 release migrasomes containing HSV-2 virions.
    2. HSV-2 in the isolated migrasomes can be transmitted to uninfected cells and cause productive infection.
    3.It is the first time that migrasomes have been found to play a role in virus spread.

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