Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice

Xiaohui Zan; Shirong Wang; Tianqi Zhang; Ying Li; Chunge Zhang; Cun Fu; Jialei Wang; Youzhi Wu; Yanhua Ma; Zhifei Li; Yan Wang; Hao Wang; Yuhai Bi; Wei Wang

doi:10.1016/j.virs.2023.09.006

December 2023

. doi: 10.1016/j.virs.2023.09.006

Citation: Xiaohui Zan, Shirong Wang, Tianqi Zhang, Ying Li, Chunge Zhang, Cun Fu, Jialei Wang, Youzhi Wu, Yanhua Ma, Zhifei Li, Yan Wang, Hao Wang, Yuhai Bi, Wei Wang. Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice .VIROLOGICA SINICA, 2023, 38(6) : 966-970. http://dx.doi.org/10.1016/j.virs.2023.09.006

Ib亚型赤羽病毒的分离鉴定及其在乳鼠上发病机制的研究

昝晓慧 ^a ,
王世荣 ^a ,
张天琦 ^a ,
李颖 ^a ,
张春鸽 ^b,d ,
付存 ^a ,
王镓磊 ^a ,
武有志 ^a ,
马艳华 ^a,e ,
李志飞 ^a ,
王岩 ^a ,
王浩 ^a ,
毕玉海 ^b,d,, ,
王炜 ^a,c,,

a. 内蒙古大学, 生命科学学院, 省部共建草原家畜生殖调控与繁育国家重点实验室, 呼和浩特市, 010070, 中国;
b. 中国科学院流感研究与预警中心, 中国科学院微生物研究所, 病原微生物与免疫学重点实验室, 北京市, 100101, 中国;
c. 内蒙古蒙微生物科技有限公司, 呼和浩特市, 012000, 中国;
d. 中国科学院大学, 北京市, 100049, 中国;
e. 内蒙古医科大学基础医学院, 呼和浩特市, 010080, 中国

通讯作者： 毕玉海, beeyh@im.ac.cn; 王炜, wangwei@imu.edu.cn
收稿日期： 2022-10-15
录用日期： 2023-09-28

摘要

迄今为止，中国分离的赤羽病病毒主要集中在Ia基因亚群和基因群II。在本研究中，我们用Vero细胞分离出了一株病毒，并通过PCR和间接免疫荧光试验确认为AKAV，命名为LK07株。LK07株与Ib基因亚群中的ISR-170/18、ISR-01、Aksu-1和Aksu-2株有较高的同源性。这是中国首次检测到赤羽病毒Ib基因亚群。为了建立哺乳小鼠的感染模型，我们将LK07病毒注射到小鼠的大脑、腹腔和皮下，并同时设立了正常对照组。脑内注射组的哺乳小鼠出现了停止吮乳、痉挛、抽搐和死亡等临床症状。组织切片结果显示脑组织局部结构疏松。qPCR和免疫组织化学结果显示所有感染组的哺乳小鼠脑组织均有阳性信号。这为进一步的AKAV分子流行病学和检测研究提供了一定的科学依据。
- 赤羽病病毒
- , 分子进化分析
- , 乳鼠

Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice

Xiaohui Zan ^a ,
Shirong Wang ^a ,
Tianqi Zhang ^a ,
Ying Li ^a ,
Chunge Zhang ^b,d ,
Cun Fu ^a ,
Jialei Wang ^a ,
Youzhi Wu ^a ,
Yanhua Ma ^a,e ,
Zhifei Li ^a ,
Yan Wang ^a ,
Hao Wang ^a ,
Yuhai Bi ^b,d,, ,
Wei Wang ^a,c,,

a. State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, 010070, China;
b. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), CAS-TWAS Center of Excellence for Emerging Infectious Diseases (CEEID), Chinese Academy of Sciences, Beijing, 100101, China;
c. Inner Mongolia Mengwei Biotech Co. Ltd, Hohhot, 012000, China;
d. University of Chinese Academy of Sciences, Beijing, 100049, China;
e. College of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010080, China

Corresponding author: Yuhai Bi, beeyh@im.ac.cn Wei Wang, wangwei@imu.edu.cn
Received Date: 15 October 2022
Accepted Date: 28 September 2023

Abstract

Highlights
1. A novel Akabane virus strain LK07 was isolated from cattle.
2. Here, we firstly reported the identification and isolation of the Akabane virus sub-lineage genogroup Ib in China.
3. The suckling mice showed clinical symptoms after intracerebrally infected with LK07 strain.
4. Viral antigen was detected in brain of mice infected with LK07 strain via IC, IP and SC routes.

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Electronic Supplementary Material

10.1016j.virs.2023.09.006-ESM.docx