. doi: 10.1016/j.virs.2023.11.001
Citation: Kexin Zong, Yuanyuan Guo, Jingdong Song, Maoshun Liu, Junfeng Hao, Jie Zhang, Xin Li, Shiyan Zhu, Shuting Huo, Ziqian Xu, Peipei Liu, Yingze Zhao, Yuhai Bi, Jiapeng Qu, George F. Gao, Jun Liu. The genomic characteristics and pathogenicity of a mammalian orthoreovirus within a new lineage from wild pika in plateau .VIROLOGICA SINICA, 2023, 38(6) : 877-888.  http://dx.doi.org/10.1016/j.virs.2023.11.001

高原野生鼠兔中新谱系哺乳动物正呼肠孤病毒的基因组特征及致病性研究

  • 近年来,来自野生动物的新发和再发病毒严重威胁着人类和家畜的健康。我们在青藏高原野生鼠兔(Ochotona curzoniae)中分离到了一株完全新型的哺乳动物正呼肠孤病毒(MRV)-Pika/MRV/GCCDC7/2019(PMRV-GCCDC7)。虽然PMRV-GCCDC7具有典型的MRV特征,其基因组十个基因片段的长度排列为3:3:4,但根据系统发育进化树分析,该病毒与其他MRV相比属于一个独立的进化分支。PMRV-GCCDC7的细胞敏感性、物种嗜性和复制动力学结果表明,该病毒可以感染4种人类来源细胞系(A549、Huh7、HCT和LoVo)和6种非人类细胞系,包括Vero-E6、LLC-MK2、BHK-21、N2a、MDCK和RfKT细胞,它们分别来自不同的哺乳动物,即猴、小鼠、犬和蝙蝠等,这表明该病毒具有感染多种宿主的潜力。BALB/c小鼠通过鼻内接种PMRV-GCCDC7感染会导致体重相对减轻、肺组织损伤和炎症,但没有出现严重的呼吸道症状和死亡。来自高原野生动物的新型呼肠孤病毒的特征扩展了我们对MRV宿主范围的认识,并提供了对其跨物种传播和动物传染病风险的进一步了解。

The genomic characteristics and pathogenicity of a mammalian orthoreovirus within a new lineage from wild pika in plateau

  • Emerging and re-emerging viruses from wild animals have seriously threatened the health of humans and domesticated animals in recent years. Herein, we isolated a new mammalian orthoreovirus (MRV), Pika/MRV/GCCDC7/2019 (PMRV-GCCDC7), in the Qinghai-Tibet Plateau wild pika (Ochotona curzoniae). Though the PMRV-GCCDC7 shows features of a typical reovirus with ten gene segments arranged in 3:3:4 in length, the virus belongs to an independent evolutionary branch compared to other MRVs based on phylogenetic tree analysis. The results of cellular susceptibility, species tropism, and replication kinetics of PMRV-GCCDC7 indicated the virus could infect four human cell lines (A549, Huh7, HCT, and LoVo) and six non-human cell lines, including Vero-E6, LLC-MK2, BHK-21, N2a, MDCK, and RfKT cell, derived from diverse mammals, i.e. monkey, mice, canine and bat, which revealed the potential of PMRV-GCCDC7 to infect a variety of hosts. Infection of BALB/c mice with PMRV-GCCDC7 via intranasal inoculation led to relative weight loss, lung tissue damage and inflammation with the increase of virus titer, but no serious respiratory symptoms and death occurred. The characterization of the new reovirus from a plateau-based wild animal has expanded our knowledge of the host range of MRV and provided insight into its risk of trans-species transmission and zoonotic diseases.

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    The genomic characteristics and pathogenicity of a mammalian orthoreovirus within a new lineage from wild pika in plateau

      Corresponding author: Jiapeng Qu, jpqu@nwipb.cas.cn
      Corresponding author: George F. Gao, gaofu@chinacdc.cn
      Corresponding author: Jun Liu, liujun@ivdc.chinacdc.cn
    • a. School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, China;
    • b. NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, 102206, China;
    • c. Laboratory Animal Center, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China;
    • d. CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China;
    • e. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, China;
    • f. Research Unit of Adaptive Evolution and Control of Emerging Viruses (2018RU009), Chinese Academy of Medical Sciences, Beijing, 100021, China

    Abstract: Emerging and re-emerging viruses from wild animals have seriously threatened the health of humans and domesticated animals in recent years. Herein, we isolated a new mammalian orthoreovirus (MRV), Pika/MRV/GCCDC7/2019 (PMRV-GCCDC7), in the Qinghai-Tibet Plateau wild pika (Ochotona curzoniae). Though the PMRV-GCCDC7 shows features of a typical reovirus with ten gene segments arranged in 3:3:4 in length, the virus belongs to an independent evolutionary branch compared to other MRVs based on phylogenetic tree analysis. The results of cellular susceptibility, species tropism, and replication kinetics of PMRV-GCCDC7 indicated the virus could infect four human cell lines (A549, Huh7, HCT, and LoVo) and six non-human cell lines, including Vero-E6, LLC-MK2, BHK-21, N2a, MDCK, and RfKT cell, derived from diverse mammals, i.e. monkey, mice, canine and bat, which revealed the potential of PMRV-GCCDC7 to infect a variety of hosts. Infection of BALB/c mice with PMRV-GCCDC7 via intranasal inoculation led to relative weight loss, lung tissue damage and inflammation with the increase of virus titer, but no serious respiratory symptoms and death occurred. The characterization of the new reovirus from a plateau-based wild animal has expanded our knowledge of the host range of MRV and provided insight into its risk of trans-species transmission and zoonotic diseases.

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