ANALYSIS BY MONOCLONAL ANTIBODY OF TOBACCO MOSIAC VIRUS DETERMINANT AND ITS ANTIGEN TYPE

ZHANG Cheng-Liang; ZHANG Zuo-Fang; JUE Xiao-Feng; CHEN Jing; HU Wei-Zhen; SONG Shu-Min; LIU Jia-Jue; SUN Yue-Ying; CHEN Bo-Quan; TIAN Bo; JI Liang

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April 1986

ZHANG Cheng-Liang, ZHANG Zuo-Fang, JUE Xiao-Feng, CHEN Jing, HU Wei-Zhen, SONG Shu-Min, LIU Jia-Jue, SUN Yue-Ying, CHEN Bo-Quan, TIAN Bo and JI Liang. ANALYSIS BY MONOCLONAL ANTIBODY OF TOBACCO MOSIAC VIRUS DETERMINANT AND ITS ANTIGEN TYPE[J]. Virologica Sinica, 1986, 1(2).

Citation: ZHANG Cheng-Liang, ZHANG Zuo-Fang, JUE Xiao-Feng, CHEN Jing, HU Wei-Zhen, SONG Shu-Min, LIU Jia-Jue, SUN Yue-Ying, CHEN Bo-Quan, TIAN Bo, JI Liang. ANALYSIS BY MONOCLONAL ANTIBODY OF TOBACCO MOSIAC VIRUS DETERMINANT AND ITS ANTIGEN TYPE .VIROLOGICA SINICA, 1986, 1(2) : 32.

应用单克隆抗体对烟草花叶毒病抗原决定簇及其抗原型的分析研究

1.
农牧渔业部植物检疫实验所
2.
中国医学科学院基础医学研究所
3.
中国预防医学中心病毒研究所
4.
中国科学院微生物研究所

摘要

收集国内9省、市、自治区14个烟草花叶病毒(TMV)分离物,在隔离条件下繁殖毒源,以同一方法,相同条件提纯并定量。以20μg/ml 为起始浓度,作10~(-1),10~(-2)、10~(-3)、10~(-4)……10~(-9)9个稀释度(设健康寄主对照),分别与适宜浓度(通过逐一测定滴度而选定的)10个 TMV 单克隆抗体(McAb)〔设多克隆抗体、正常细胞腹水(SP2/0)对照〕进行 ELISA 试验,结果从14株分离 TMV 分离物中识别到8种抗原决定簇,并将这些 TMV 分离物划分为六个抗原型。这揭示了应用 McAb 区分 TMV 株系是一个很有希望的方法

ANALYSIS BY MONOCLONAL ANTIBODY OF TOBACCO MOSIAC VIRUS DETERMINANT AND ITS ANTIGEN TYPE

1.
1.Institute of plant Quarantine
2.
Ministry of Agriculture
3.
Animal Husbandry and Fishery
4.
Institute of Microbiology Academia Sinica.

Abstract

Fourteen isolates of Tobacco Mosiac Virus(TMV)were collected from 9 different regions of China.They were multiplied under isolating con- ditions and purified and measured by the same method.The concentra- tion series were started from 20μg/ml and then were diluted into nine dilutions from 10~(-1).10~(-2)…to 10~(-9)(contrasted with healthy host).Each was reacted individually with 10 TMV monoclonal antibodies of optimal dilution(selected through detecting titers)in ELISA(contrasted with poly- clonal antibody ...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

ANALYSIS BY MONOCLONAL ANTIBODY OF TOBACCO MOSIAC VIRUS DETERMINANT AND ITS ANTIGEN TYPE

1. 1.Institute of plant Quarantine
2. Ministry of Agriculture
3. Animal Husbandry and Fishery
4. Institute of Microbiology Academia Sinica.

Abstract: Fourteen isolates of Tobacco Mosiac Virus(TMV)were collected from 9 different regions of China.They were multiplied under isolating con- ditions and purified and measured by the same method.The concentra- tion series were started from 20μg/ml and then were diluted into nine dilutions from 10~(-1).10~(-2)…to 10~(-9)(contrasted with healthy host).Each was reacted individually with 10 TMV monoclonal antibodies of optimal dilution(selected through detecting titers)in ELISA(contrasted with poly- clonal antibody ...