INFLUENCE OF DIFFERENT TEMPERATURES ON THE REPLICATION OF BUZURA SUPPRESSARIA NPV IN OVARY CELL LINE

WANG Lu-Ming; Zhang-Yang-Lian; XIE Tian-En

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August 1986

WANG Lu-Ming, Zhang-Yang-Lian and XIE Tian-En. INFLUENCE OF DIFFERENT TEMPERATURES ON THE REPLICATION OF BUZURA SUPPRESSARIA NPV IN OVARY CELL LINE[J]. Virologica Sinica, 1986, 1(4).

Citation: WANG Lu-Ming, Zhang-Yang-Lian, XIE Tian-En. INFLUENCE OF DIFFERENT TEMPERATURES ON THE REPLICATION OF BUZURA SUPPRESSARIA NPV IN OVARY CELL LINE .VIROLOGICA SINICA, 1986, 1(4) : 80.

不同温度对油桐尺蠖核型多角体病毒在卵巢细胞系中增殖的影响

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

本试验用油桐尺蠖核型乡角体病毒(Buzura Suppressaria Nuclear Polyhedro-sis Virus)感染油桐尺蠖卵巢细胞系,分别置于0℃、15℃、20℃、26℃、28℃、30℃、37℃下静止培养,观察细胞病理变化,测定细胞的感染百分率和多角体含量。试验结果表明,不同温度对病毒的感染率及其在细胞中复制有明显的影响。26℃是油桐尺蠖核型多角体病毒感染卵巢细胞并形成多角体的最适培养温度,感染率最高,多角体含量也最多。培养温度过低或过高,多角体都不能在细胞内复制。

INFLUENCE OF DIFFERENT TEMPERATURES ON THE REPLICATION OF BUZURA SUPPRESSARIA NPV IN OVARY CELL LINE

1.
Wuhan Institute of Virology Academia Sinica
2.
Wuhan

Abstract

This experiment is basad on the infection of Buzura Suppressaria ovary cell line by Buzura Suppressaria NPV. After static incubation of the infected cell line at different temperatures. 0℃, 15℃, 20℃, 26℃, 28℃,30℃, and 37℃, the cytopathologieal effect have been observed by the determination of thepercentage of the infected cells and the yield of polyhedra. The result has been shown that the infection rate of the virus during their replication in cells is obviously influence at different temperature. The opti...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

INFLUENCE OF DIFFERENT TEMPERATURES ON THE REPLICATION OF BUZURA SUPPRESSARIA NPV IN OVARY CELL LINE

1. Wuhan Institute of Virology Academia Sinica
2. Wuhan

Abstract: This experiment is basad on the infection of Buzura Suppressaria ovary cell line by Buzura Suppressaria NPV. After static incubation of the infected cell line at different temperatures. 0℃, 15℃, 20℃, 26℃, 28℃,30℃, and 37℃, the cytopathologieal effect have been observed by the determination of thepercentage of the infected cells and the yield of polyhedra. The result has been shown that the infection rate of the virus during their replication in cells is obviously influence at different temperature. The opti...