LI Fang-He, CHEN Hong-Yun, YU Zhi-Qun, LI Yu-Xue and HAO Lian-Jie. DETECTION OF IgM ANTI-HBc BY ENZYME LINKED IMMUNOSORBENT ASSAY WITH AVIDIN LABELLED PEROXIDASE AND BIOTIN LABELLED ANTI-HBc[J]. Virologica Sinica, 1987, 2(3).
Citation:
LI Fang-He, CHEN Hong-Yun, YU Zhi-Qun, LI Yu-Xue, HAO Lian-Jie.
DETECTION OF IgM ANTI-HBc BY ENZYME LINKED IMMUNOSORBENT ASSAY WITH AVIDIN LABELLED PEROXIDASE AND BIOTIN LABELLED ANTI-HBc .VIROLOGICA SINICA, 1987, 2(3)
: 28.
标记抗生物素-生物素酶联免疫吸附试验(LABELISA)检测血清抗-HBc/IgM的研究
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同济医科大学附属同济医院临床免疫研究室
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同济医科大学附属同济医院临床免疫研究室 武汉
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摘要
本文将BAS用于固相免疫吸附试验,建立LAB-ELISA抗-HBc/IgM试验方法,并与普通ELISA法进行了比较。实验表明:本法敏感性较普通ELISA高2—4倍,检出率高出后者6.8％,且具有较好的重复性。为血清抗-HBc/IgM检测提供了一种新的、切实可行的方法。本试验采用改良过碘酸盐法制备酶标记抗生物素,以含A蛋白葡萄球菌菌体亲和吸附试验制备抗-μ抗体,在稀释液中加入凝聚正常兔IgG以克服类风湿因子的干扰、考核方法的特异性采用了含A蛋白葡萄球菌菌体吸附试验及抗HBc-抑制试验。
DETECTION OF IgM ANTI-HBc BY ENZYME LINKED IMMUNOSORBENT ASSAY WITH AVIDIN LABELLED PEROXIDASE AND BIOTIN LABELLED ANTI-HBc
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Abstract
A new immunoenzymatic assay was established with avidin labelled peroxidase(A-P) and biotin labelled anti-HBc(B-Anti-HBc)for deteeting IgM antibody to hepatitis B core antigen. A-P, B-Anti-HBc and antiserum to human μ chain for the assay were prepared by our laboratory, Aggregated normal rabbit IgG was added to the buffer solution employed as a dilution to overcome the influence of rheumatoid factor of the blood specimens The specificity of the method was proved by SPA absorption method and anti-HBc inhibit...
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