HUMAN CYTOMECALOVIRUS LATENCY IN HUMAN EMBRYONIC FORESKIN CELLS IN VITRO

ZENG Fan-Ji

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June 1987

ZENG Fan-Ji. HUMAN CYTOMECALOVIRUS LATENCY IN HUMAN EMBRYONIC FORESKIN CELLS IN VITRO[J]. Virologica Sinica, 1987, 2(3).

Citation: ZENG Fan-Ji. HUMAN CYTOMECALOVIRUS LATENCY IN HUMAN EMBRYONIC FORESKIN CELLS IN VITRO .VIROLOGICA SINICA, 1987, 2(3) : 34.

人巨细胞病毒在包皮细胞培养中的潜伏性感染

曾凡济

1.
武汉大学病毒系武汉

摘要

用具有特异性抑制蛋白质糖基化作用的含氨基葡糖的衣霉素Tunicamycin(TM)处理人胚包皮细胞(HEF)建立起人巨细胞病毒(HCMV)的潜伏性感染模型。观察了TM对HEF细胞及HCMV的毒性作用,当TM浓度大于500ng/ml时对单层细胞生长有影响;在TM浓度为500ng/ml时,细胞生长虽受一定程度的影响,但仍然逐步生长扩增;不同浓度的TM对HCMV有不同程度的影响,但不能将全部HCMV在短期内完全抑制,在TM含量1000ng/ml的维持液中处理5日的HCMV感染细胞培养物中未检出感染性病毒。感染HCMV的HEF单层细胞保持在含TM500ng/ml的维持液中于37℃培养7日,然后换加不含TM的维持液并改置40.5℃中培养,可保持HCMV以非感染性的病毒状态潜伏于HEF细胞内长达135日;在此潜伏期间如将细胞培养物由40.5℃再移置37℃中培养,HCMV能被激活为可检测到高滴度的感染性病毒。定期取在40.5℃中培养的HCMV潜伏感染细胞培养物进行感染中心测定(Infections Center Assays),感染中心滴度(Infectous Center Titer)为0.0004％～0.005％,只有...

HUMAN CYTOMECALOVIRUS LATENCY IN HUMAN EMBRYONIC FORESKIN CELLS IN VITRO

ZENG Fan-Ji

1.
Department of Virology

Abstract

Human cytomegalovirus(HCMV)latency model in human embryonic foreskin cells(HEF)was established with 500ng/ml of Tunicamycin(TM) which is a glucosamine-containing antibiotic that specifically inhibits glycosylation of proteins. Toxic effects in confluent monolayer cultures occurred when more than 500ng/ml of TM was used. The different concentrations of TM reduced the yield of HCMV to varying degrees, however, HCMV was not totally inhibited. At 5 days postinfection HCMV was not detectable at a TM concentratio...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

HUMAN CYTOMECALOVIRUS LATENCY IN HUMAN EMBRYONIC FORESKIN CELLS IN VITRO

ZENG Fan-Ji

1. Department of Virology

Abstract: Human cytomegalovirus(HCMV)latency model in human embryonic foreskin cells(HEF)was established with 500ng/ml of Tunicamycin(TM) which is a glucosamine-containing antibiotic that specifically inhibits glycosylation of proteins. Toxic effects in confluent monolayer cultures occurred when more than 500ng/ml of TM was used. The different concentrations of TM reduced the yield of HCMV to varying degrees, however, HCMV was not totally inhibited. At 5 days postinfection HCMV was not detectable at a TM concentratio...