ELECTRON MICROSCOPIC STUDIES ON DNA FROM A DENSONUCLEOSIS VIRUS(YAMANASHI ISOLATE) OF THE SILKWORM, BOMBYX MORI

HU Yuan-Yang; H.SEKI; S. KAWASE

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June 1987

HU Yuan-Yang, H.SEKI and S. KAWASE. ELECTRON MICROSCOPIC STUDIES ON DNA FROM A DENSONUCLEOSIS VIRUS(YAMANASHI ISOLATE) OF THE SILKWORM, BOMBYX MORI[J]. Virologica Sinica, 1987, 2(3).

Citation: HU Yuan-Yang, H.SEKI, S. KAWASE. ELECTRON MICROSCOPIC STUDIES ON DNA FROM A DENSONUCLEOSIS VIRUS(YAMANASHI ISOLATE) OF THE SILKWORM, BOMBYX MORI .VIROLOGICA SINICA, 1987, 2(3) : 94.

家蚕浓核病毒(山梨株)DNA的电子显微镜研究

1.
武汉大学病毒系
2.
日本山梨县蚕业试验场
3.
日本名古屋大学农学部武汉

摘要

最近所分离的家蚕浓核病毒(DNV)山梨株(Y-DNV)与最初在家蚕中发现的DNV伊那株在各种理化性质方面有所不同。从Y-DNV中提取的病毒DNA在琼脂糖凝胶电泳中出现二条带(DNA Ⅰ和Ⅱ)。用限制性内切酶处理这些DNA,其结果也明显不同。为了弄清这些现象,用电子显微镜对这些DNA分子进行研究。通过琼脂糖凝胶电泳分离的DNA Ⅰ和DNA Ⅱ都是双链线状分子,但DNA Ⅰ分子的长度略长。在电子显微镜下,二种DNA的构象没有差异,因此可以肯定DNA Ⅰ和DNAⅡ有不同的碱基序列组成。这在DNV中是罕见的,因为到目前为止所知道的所有DNV都只有4种结构蛋白。基于这种情况,在前一篇论文我们曾推测这些蛋白质可能是来自两种DAV,它们各自具有其中3种或4种结构蛋白。应当指出的是,Y-DNV的衣壳本身是由一种还是由二种结构所组成的还需要进一步的研究给予证实。因为Y-DNV的病毒直径很小,它不可能包含二种DNA(分子量分别为2.1×10~6d,和1.7×10~6d.)所以家蚕Y-DNV很可能是由二种相似但是不相同的DNV所组成的混合物。

ELECTRON MICROSCOPIC STUDIES ON DNA FROM A DENSONUCLEOSIS VIRUS(YAMANASHI ISOLATE) OF THE SILKWORM, BOMBYX MORI

1.
1 Department of Virology
2.
Wuhan University
3.
Wuhan 2 Yamanashi-ken Sericultural Experiment Station

Abstract

Chemical properties of newly isolated Bombyx densonucleosis virus(BmDNV), Yamanashi isolate(Y-DNV), were found to be different from those of first isolated BmDNV, Ina isolate. The viral DNA of Y-DNV was resolved in an agarose gel electropherogram into two closely migrating bands, DNA Ⅰ and Ⅱ. The digests of these DNAs treated with some restriction enzymes differed clearly from each other. In order to clarify this phenomenon, the structure of these DNA molecules was investigated by electron microscopy. Both ...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

ELECTRON MICROSCOPIC STUDIES ON DNA FROM A DENSONUCLEOSIS VIRUS(YAMANASHI ISOLATE) OF THE SILKWORM, BOMBYX MORI

1. 1 Department of Virology
2. Wuhan University
3. Wuhan 2 Yamanashi-ken Sericultural Experiment Station

Abstract: Chemical properties of newly isolated Bombyx densonucleosis virus(BmDNV), Yamanashi isolate(Y-DNV), were found to be different from those of first isolated BmDNV, Ina isolate. The viral DNA of Y-DNV was resolved in an agarose gel electropherogram into two closely migrating bands, DNA Ⅰ and Ⅱ. The digests of these DNAs treated with some restriction enzymes differed clearly from each other. In order to clarify this phenomenon, the structure of these DNA molecules was investigated by electron microscopy. Both ...