STUDIES ON A METHOD FOR QUANTITATIVE DETECTING OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE

MO Yin-Yuan; REN Xin-Zheng; FANG Zhong-Da

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August 1987

MO Yin-Yuan, REN Xin-Zheng and FANG Zhong-Da. STUDIES ON A METHOD FOR QUANTITATIVE DETECTING OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE[J]. Virologica Sinica, 1987, 2(4).

Citation: MO Yin-Yuan, REN Xin-Zheng, FANG Zhong-Da. STUDIES ON A METHOD FOR QUANTITATIVE DETECTING OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE .VIROLOGICA SINICA, 1987, 2(4) : 73.

噬菌体定量检测青枯假单胞杆菌(Pseudomonas solanacearum)方法的研究——Ⅱ 噬菌体ZP-2对土壤含菌量测定的研究

1.
南京农业大学植病组
2.
南京农业大学植病组南京

摘要

采用噬菌体技术对人工接种土壤的青枯假单胞杆菌姜瘟菌株进行测定,结果表明,在每克土含10~3菌落形成单位(CFU)的情况下,回收率为57.4％,检测的敏感性较高,在每克土含10~2(CFU)的条件下也能测到活菌数。因此,这种方法是一种快速、简便、有前途的方法。

STUDIES ON A METHOD FOR QUANTITATIVE DETECTING OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE

1.
Department of Plant Protection
2.
Nanjing Agricultural University

Abstract

The present paper deals with the phaga technique to quantitative determinate the survival of causing bacteria of ginger wilt(Pseudomonas solanacearum)in soil. Results show that the bacteria in inoculated soil could recover to 57.4％ at 10~3 CFU/g soil. The living bacterial cells,even at 10~2CFU/g soil,could be detected. The phage technique is a sensitive, rapid and simple method.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

STUDIES ON A METHOD FOR QUANTITATIVE DETECTING OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE

1. Department of Plant Protection
2. Nanjing Agricultural University

Abstract: The present paper deals with the phaga technique to quantitative determinate the survival of causing bacteria of ginger wilt(Pseudomonas solanacearum)in soil. Results show that the bacteria in inoculated soil could recover to 57.4％ at 10~3 CFU/g soil. The living bacterial cells,even at 10~2CFU/g soil,could be detected. The phage technique is a sensitive, rapid and simple method.