The Enzyme-Linked Immunoelectrotransfer Blot ("Western-Blot"): Technical Considerations

V.C.W. Tsang; CHEN Ming-Bin

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February 1988

V.C.W. Tsang and CHEN Ming-Bin. The Enzyme-Linked Immunoelectrotransfer Blot ('Western-Blot'): Technical Considerations[J]. Virologica Sinica, 1988, 3(1).

Citation: V.C.W. Tsang, CHEN Ming-Bin. The Enzyme-Linked Immunoelectrotransfer Blot ("Western-Blot"): Technical Considerations .VIROLOGICA SINICA, 1988, 3(1) : 3.

酶联印迹法(Western-Blot)应考虑的几个技术要素

V.C.W. Tsang ,
陈明彬

摘要

本文对蛋白质的十二烷基硫酸钠(SDS)-聚丙烯酰胺凝胶电泳(PAGE),酶联印迹法(EITB)的条件和所用试剂的改进进行了讨论。EITB的应用已在各种研究和日常临床检测得到证实。文章着重阐明有关印迹的条件、灵敏度、试剂和仪器的使用局限性、样品的处理和加样诸方面的问题。

The Enzyme-Linked Immunoelectrotransfer Blot ("Western-Blot"): Technical Considerations

Abstract

(Helminthic Immunology Activity, Helminthic Diseases Branch, Divison of Parasitic Diseases, Center for Infectious Diseases, Centers for Disease Control, U. S. Public Health Service, Department of Health and Human Services, Atlanta, GA 30333 USA)

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沈阳化工大学材料科学与工程学院沈阳 110142

The Enzyme-Linked Immunoelectrotransfer Blot ("Western-Blot"): Technical Considerations

Abstract: (Helminthic Immunology Activity, Helminthic Diseases Branch, Divison of Parasitic Diseases, Center for Infectious Diseases, Centers for Disease Control, U. S. Public Health Service, Department of Health and Human Services, Atlanta, GA 30333 USA)