Analysis of the Structural Polypeptides of Nuclear Polyhedrosis Virus From Buzura suppressaria Guenee

LIU Nian-Cui; MENG Xiao-Lin

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February 1988

LIU Nian-Cui and MENG Xiao-Lin. Analysis of the Structural Polypeptides of Nuclear Polyhedrosis Virus From Buzura suppressaria Guenee[J]. Virologica Sinica, 1988, 3(1).

Citation: LIU Nian-Cui, MENG Xiao-Lin. Analysis of the Structural Polypeptides of Nuclear Polyhedrosis Virus From Buzura suppressaria Guenee .VIROLOGICA SINICA, 1988, 3(1) : 48.

油桐尺蠖核型多角体病毒结构多肽的分析

刘年翠 ,
孟小林

1.
武汉大学病毒系
2.
武汉大学病毒系武汉

摘要

本文采用不连续系统的垂直板SDS—PAGE对油桐尺蠖核型多角体病毒(Buzura suppressaria Guenee Nuclear Polyhedrosis Virus简称BsNPV)的多角体蛋白、病毒粒子的结构多肽进行了分析。结果表明,经EDTA和热处理的多角体蛋白仅有一条主带,其分子量为31×10~3±1000d;病毒粒子至少有23种结构多肽,其分子量的范围在13—107×10~3d之间。用PAS染色测定多角体蛋白、病毒粒子中的糖蛋白,结果呈阴性反应。

Analysis of the Structural Polypeptides of Nuclear Polyhedrosis Virus From Buzura suppressaria Guenee

1.
Department of Virology
2.
Wuhan University

Abstract

The structural polypeptides of Buzura suppressaria Guenee nuclear polyhedrosis virus was studied by vertical slab polyacrylamide gel electrophoresis. The results revealed that the polyhedrin—the only major polypeptide with molecular weight of 31×10~3±1000d and the virus particle had at least 23 bands with molecular weight ranging from 13 to 107×10~3d. Glycoproteins of polyhedrin and virus particle were absent by PAS staining detection.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of the Structural Polypeptides of Nuclear Polyhedrosis Virus From Buzura suppressaria Guenee

1. Department of Virology
2. Wuhan University

Abstract: The structural polypeptides of Buzura suppressaria Guenee nuclear polyhedrosis virus was studied by vertical slab polyacrylamide gel electrophoresis. The results revealed that the polyhedrin—the only major polypeptide with molecular weight of 31×10~3±1000d and the virus particle had at least 23 bands with molecular weight ranging from 13 to 107×10~3d. Glycoproteins of polyhedrin and virus particle were absent by PAS staining detection.