Assay for HSV-Induced Deoxythymidine Kinase and Its Application to Antiviral Compound Study

TANG Hua; LIN Zhong-Yuan

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April 1988

TANG Hua and LIN Zhong-Yuan. Assay for HSV-Induced Deoxythymidine Kinase and Its Application to Antiviral Compound Study[J]. Virologica Sinica, 1988, 3(2).

Citation: TANG Hua, LIN Zhong-Yuan. Assay for HSV-Induced Deoxythymidine Kinase and Its Application to Antiviral Compound Study .VIROLOGICA SINICA, 1988, 3(2) : 148.

HSV诱导的dTK分析及其在抗病毒药物研究中的应用

汤华 ,
任中原

1.
天津医学院微生物学教研室
2.
天津医学院微生物学教研室天津

摘要

本试验描述了以~(125)I—UdR为基质检测dTK活性的方法。发现HSV—1,HSV—2在感染的HeLa细胞中诱导产生了高活性的dTK,同时证明了病毒dTK对新的抗病毒化合物——DHPG存在较高的敏感性,并讨论了其敏感性与DHPG抗病毒作用的关系及其捡测dTK的可能意义。

Assay for HSV-Induced Deoxythymidine Kinase and Its Application to Antiviral Compound Study

TANG Hua ,
LIN Zhong-Yuan

1.
Department of Microbiology
2.
Tianjin Medical College

Abstract

An assay for herpes simplex virus type 1 and 2 induced deoxythymidine (dTK) was described by using ~(125)Ⅰ-UdR as substrate. The assay was also used for detecting the sensitivity of HSV-1 and HSV-2 induced dTK to DHPG. The result showed that sensitivity of viral dTK to DHPG was higher than HeLa cell-dTK s. In addition, we thought that toxicity of DHPG to host cells might be related to the low sensitivity of cell-dTK to DHPG.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Assay for HSV-Induced Deoxythymidine Kinase and Its Application to Antiviral Compound Study

TANG Hua ,
LIN Zhong-Yuan

1. Department of Microbiology
2. Tianjin Medical College

Abstract: An assay for herpes simplex virus type 1 and 2 induced deoxythymidine (dTK) was described by using ~(125)Ⅰ-UdR as substrate. The assay was also used for detecting the sensitivity of HSV-1 and HSV-2 induced dTK to DHPG. The result showed that sensitivity of viral dTK to DHPG was higher than HeLa cell-dTK s. In addition, we thought that toxicity of DHPG to host cells might be related to the low sensitivity of cell-dTK to DHPG.