Study of Cytopathic Effects and Reproduction of Epidemic Hemorrhagic Fever Virus(EHFV) in Human Lymphocyte Cell Line

SHEN Hong-Kai; YAO Gai-Xin

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April 1988

SHEN Hong-Kai and YAO Gai-Xin. Study of Cytopathic Effects and Reproduction of Epidemic Hemorrhagic Fever Virus(EHFV) in Human Lymphocyte Cell Line[J]. Virologica Sinica, 1988, 3(2).

Citation: SHEN Hong-Kai, YAO Gai-Xin. Study of Cytopathic Effects and Reproduction of Epidemic Hemorrhagic Fever Virus(EHFV) in Human Lymphocyte Cell Line .VIROLOGICA SINICA, 1988, 3(2) : 173.

流行性出血热病毒(EHFV)在传代人淋巴细胞上的致病变作用增殖的研究

沈宏开 ,
姚盖新

1.
安徽省医学科学研究所病毒室
2.
安徽省医学科学研究所病毒室合肥

摘要

流行性出血热病毒陈株、L_99和SR_11株按种传代淋巴细胞克隆株Ly-A_6后第5～7日观察到CPE,开始时受感染细胞浆内颗粒增多,细胞圆缩、聚积成堆,随后细胞膜模糊不清,2～3天脱落,同时制片IFA检测到胞浆内特异性荧光颗粒,以上结果能被EHF免疫血清所中和。接种病毒后的细胞单层用l％甲基纤维素加维持液覆盖,第6日吸去覆盖液,加5％甲醛-1％结晶紫固定染色可见到明显的PFU。EHFV致病变细胞株的染得将为病原学研究提供了优越的基质细胞。

Study of Cytopathic Effects and Reproduction of Epidemic Hemorrhagic Fever Virus(EHFV) in Human Lymphocyte Cell Line

1.
Department of Virology
2.
Anhui Institute of Medical Sciences

Abstract

A human lymphocyte cell line was inoculated with Chen, L99, and SR11 strains of EHF virus respectively. Cytopathic effects(CPE) were observed for 5—7 days after inoculation. First of all, the cells granulated and rounded up, then cell degeneration occured rapidly. The clustered and clumed cells still adhered cn the glass surface without forming syncytium. Some 48—72 hours after the CPE appeared, the cells fell off completely from the glass surface. Specific immunoflorescent particles were examined in the cy...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study of Cytopathic Effects and Reproduction of Epidemic Hemorrhagic Fever Virus(EHFV) in Human Lymphocyte Cell Line

1. Department of Virology
2. Anhui Institute of Medical Sciences

Abstract: A human lymphocyte cell line was inoculated with Chen, L99, and SR11 strains of EHF virus respectively. Cytopathic effects(CPE) were observed for 5—7 days after inoculation. First of all, the cells granulated and rounded up, then cell degeneration occured rapidly. The clustered and clumed cells still adhered cn the glass surface without forming syncytium. Some 48—72 hours after the CPE appeared, the cells fell off completely from the glass surface. Specific immunoflorescent particles were examined in the cy...