TU Zheng-Jin and YU Shan-Qian. Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus(RMVsh) Ⅱ Methods of Immunological Reaction of Monoelonal Antibodies and its Applying in the Differentiation of Virus Strains[J]. Virologica Sinica, 1988, 3(4).
Citation:
TU Zheng-Jin, YU Shan-Qian.
Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus(RMVsh) Ⅱ Methods of Immunological Reaction of Monoelonal Antibodies and its Applying in the Differentiation of Virus Strains .VIROLOGICA SINICA, 1988, 3(4)
: 376.
长叶车前花叶病毒上海分离株单克隆抗体的制备及其免疫学特性——Ⅱ单克隆抗体免疫反应方法及在株系鉴别上的应用
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摘要
采用两种不同的ELISA方法比较了长叶车前花叶病毒上海分离株(RMVsh)7种单克隆抗体对完整病毒及其外壳蛋白的反应。结果表明不同的单克隆抗体在两种ELISA方法中的反应特性各异。这可能由于ELISA方法对抗原结构的影响而导致抗原抗体结合的不同。比较烟草花叶病毒群的7个分离株对RMVsh单克隆抗体和兔多克隆抗体的反应。结果表明单克隆抗体能与属于RMV的3个分离株起反应,并能将它们区分开来,与属于TMV的4个分离株均无反应。而兔多克隆抗体与这7个分离株均有较强的反应,但难以区分各株系。表明单克隆抗体在株系鉴别上具有高度的特异性。
Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus(RMVsh) Ⅱ Methods of Immunological Reaction of Monoelonal Antibodies and its Applying in the Differentiation of Virus Strains
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Department of Biology
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Fudan University
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Abstract
The reactions of seven monoclonal antibodies specific for Shanghai Isolate of Ribgrass Mosaic Virus (RMVsh) with virion and its coat protein by two different ELISA methods were compared. The results showed that the properties of reaction of some monoclonal antibodies in two different ELISA methods were different. It may be that the antigen structure was effected by the different ELISA methods, so that the capability of the binding of antigen to antibody was changed. The results of determination of seven str...
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