Detection of Human Papillomavirus and Herpes Simplex Virus Type-2 DNA Sequences in Cervical Lesions

MENG Xiang-Jin; SUN Yu; LIU Zhi-Hui; CHEN Min-Hui; LI Xin-Zhi; ZHANG Wei-Xin; LI Hun; HAN Ri-Cai; CI Jing-Yi; ZHANG Wei; CENG Wei-Kun; HU Lun-Ying; NONG Shu-Gui

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February 1989

MENG Xiang-Jin, SUN Yu, LIU Zhi-Hui, CHEN Min-Hui, LI Xin-Zhi, ZHANG Wei-Xin, LI Hun, HAN Ri-Cai, CI Jing-Yi, ZHANG Wei, CENG Wei-Kun, HU Lun-Ying and NONG Shu-Gui. Detection of Human Papillomavirus and Herpes Simplex Virus Type-2 DNA Sequences in Cervical Lesions[J]. Virologica Sinica, 1989, 4(1).

Citation: MENG Xiang-Jin, SUN Yu, LIU Zhi-Hui, CHEN Min-Hui, LI Xin-Zhi, ZHANG Wei-Xin, LI Hun, HAN Ri-Cai, CI Jing-Yi, ZHANG Wei, CENG Wei-Kun, HU Lun-Ying, NONG Shu-Gui. Detection of Human Papillomavirus and Herpes Simplex Virus Type-2 DNA Sequences in Cervical Lesions .VIROLOGICA SINICA, 1989, 4(1) : 22.

宫颈疾患中人乳头瘤病毒和疱疹病毒Ⅱ型DNA的检测

1.
湖北医学院病毒研究所
2.
中国医学科学院基础所
3.
湖北医学院附二医院妇产科
4.
湖北医学院附二医院妇产科武汉

摘要

本文应用HPV11,16,18型和HSV-2N/BglⅡ、HSV-2L/HindⅢDNA片段等五个分子探针,通过斑点杂交技术对79例宫颈疾患(包括50例宫颈癌和29例宫颈糜烂)组织DNA进行了检测,结果发现宫颈癌组织HPV16,18和11的阳性率分别为44％,12％和4％,而宫颈糜烂组织中HPV16,18和11的阳性率分别为14％,7％和14％;且3例标本HPV16和HPV18均呈弱杂交反应;在被检的所有宫颈癌组织中各有2例分别与HSV-2N/BglⅡHSV-2L/HindⅢ弱杂交,宫颈糜烂组织无一例阳性。结果提示,HPV在宫颈癌的发生过程中可能起主要作用,HSV-2的作用尚不确定,可能与HPV起协同作用。
- 分子杂交
- , 人乳头瘤病毒
- , 疱疹病毒Ⅱ型
- , 宫颈疾患

Detection of Human Papillomavirus and Herpes Simplex Virus Type-2 DNA Sequences in Cervical Lesions

1.
Institute of Virology
2.
Hubei Medical College
3.
WuhanInstitute of Basic Medical Science
4.
Chinese Academy of Medical Science

Abstract

79 cervical lesions(50 cervical cancer and 29 cervical erosion) were studied by dot hybridization using five DNA probes: HPV 16, 18, 11 and HSV-2N/BglⅡ, HSV-2 L/HindⅢ. Among them, the positive percentage of HPV 16, 18 and 11 in cervical carcinoma was 44%, 12% and 4%, respectively; but 14%, 7%, and 14% in cervical erosion, respectively; and 3 samples showed weakly cross-hybridization between HPV 16 and HPV 18. of all cervical carcinoma studied, only 2 hybridize, weakly with HSV-2 N/BglⅡ and HSV-2 L/HindⅢ DNA...
- Molecular Hybridization
- , Human Papilloma virus
- , Herpes Simplex Virus-2
- , Cervical Lesions

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Human Papillomavirus and Herpes Simplex Virus Type-2 DNA Sequences in Cervical Lesions

1. Institute of Virology
2. Hubei Medical College
3. WuhanInstitute of Basic Medical Science
4. Chinese Academy of Medical Science

Abstract: 79 cervical lesions(50 cervical cancer and 29 cervical erosion) were studied by dot hybridization using five DNA probes: HPV 16, 18, 11 and HSV-2N/BglⅡ, HSV-2 L/HindⅢ. Among them, the positive percentage of HPV 16, 18 and 11 in cervical carcinoma was 44%, 12% and 4%, respectively; but 14%, 7%, and 14% in cervical erosion, respectively; and 3 samples showed weakly cross-hybridization between HPV 16 and HPV 18. of all cervical carcinoma studied, only 2 hybridize, weakly with HSV-2 N/BglⅡ and HSV-2 L/HindⅢ DNA...