Rapid Identification of Dengue Virus Isolated by Using Monoclonal Antibodies in an Indirect Immunofluorescence Assay

LIN Bi-Hu; LIU Le-He; ZHOU Zhen-Jian; CHEN Pei-Jia; LIN Hai; LU Xiao-Dong; MAI Guang-Da

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February 1989

LIN Bi-Hu, LIU Le-He, ZHOU Zhen-Jian, CHEN Pei-Jia, LIN Hai, LU Xiao-Dong and MAI Guang-Da. Rapid Identification of Dengue Virus Isolated by Using Monoclonal Antibodies in an Indirect Immunofluorescence Assay[J]. Virologica Sinica, 1989, 4(1).

Citation: LIN Bi-Hu, LIU Le-He, ZHOU Zhen-Jian, CHEN Pei-Jia, LIN Hai, LU Xiao-Dong, MAI Guang-Da. Rapid Identification of Dengue Virus Isolated by Using Monoclonal Antibodies in an Indirect Immunofluorescence Assay .VIROLOGICA SINICA, 1989, 4(1) : 34.

应用型特异性单克隆抗体快速鉴定登革病毒

1.
海南大学医学部微生物学教研室
2.
中山医科大学微生物学与免疫学教研室病毒室
3.
海口市人民医院检验科

摘要

1986年,在海南岛发生登革热爆发流行期间,应用型特异性单克隆抗体以间接免疫荧光法快速鉴定所分离的35株登革热毒。接种标本的第一代C_6/36细胞于感染病毒后96小时内登革病毒抗原检出率可达97％
- 登革热
- , 型持异性单克隆抗体
- , Ⅱ型登革病毒
- , 间接免疫荧光

Rapid Identification of Dengue Virus Isolated by Using Monoclonal Antibodies in an Indirect Immunofluorescence Assay

1.
1. Hai Nan University
2.
Medical college
3.
Department of Microbiology

Abstract

With the microcullured by C_6/36 clone of Aedes Albopictus cells, 35 dengue virus strain were isolated from patients with dengue fever in Hainan island. These virus were identified as type 2 virus by means of four dengue virus type-specific monoclonal antibody in an indirect immunofluorescene assay. The virus antigen can be detected from postinfectious of C_6/36 cells within 48hr.as a result of using dengue type-specific McAb.
- Dengue fever
- , Type-specific monoclonal antibody
- , Type 2 dengue virus
- , indirect immunofluorescene assay

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Rapid Identification of Dengue Virus Isolated by Using Monoclonal Antibodies in an Indirect Immunofluorescence Assay

1. 1. Hai Nan University
2. Medical college
3. Department of Microbiology

Abstract: With the microcullured by C_6/36 clone of Aedes Albopictus cells, 35 dengue virus strain were isolated from patients with dengue fever in Hainan island. These virus were identified as type 2 virus by means of four dengue virus type-specific monoclonal antibody in an indirect immunofluorescene assay. The virus antigen can be detected from postinfectious of C_6/36 cells within 48hr.as a result of using dengue type-specific McAb.