Study of Attenuation of Dengue Type 4 Virus by Consecutive Passages in Primary Hamster Kidney Tissue Culture

LIU Li-Hua; YU Yong-Xin; LI Zheng-Ping

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February 1989

LIU Li-Hua, YU Yong-Xin and LI Zheng-Ping. Study of Attenuation of Dengue Type 4 Virus by Consecutive Passages in Primary Hamster Kidney Tissue Culture[J]. Virologica Sinica, 1989, 4(1).

Citation: LIU Li-Hua, YU Yong-Xin, LI Zheng-Ping. Study of Attenuation of Dengue Type 4 Virus by Consecutive Passages in Primary Hamster Kidney Tissue Culture .VIROLOGICA SINICA, 1989, 4(1) : 38.

登革热Ⅳ型病毒在地鼠肾细胞培养内减毒的研究

1.
中国药品生物制品检定所虫媒病毒专业实验室
2.
中国药品生物制品检定所虫媒病毒专业实验室北京

摘要

本文报道登革热Ⅳ型病毒Ban18株通过原代地鼠肾细胞连续传代后的适应和减毒过程。 Ban18原株对原代地鼠肾细胞无病变或仅有极轻微病变,对乳小白鼠及断乳鼠的脑内毒力高达LD_(50)为Log6—7。通过连续传20—70代后,病变逐渐加重,出现时间缩短,二天即可达到细胞完全破坏。培养液内的病毒含量也随之提高达10~6—10~7/ml TCID_(50)。但随着传代代数增加,病毒对乳小白鼠的嗜神经毒力逐渐降低,脑内接种后仅个别发病或完全不致死。脑组织病理变化也较原株明显减轻,接种树鼩不产生病毒血症。以上结果符合弱毒株的减毒指标,是一株有希望的活疫苗毒株。
- 登革热Ⅳ型病毒
- , 地鼠肾细胞减毒

Study of Attenuation of Dengue Type 4 Virus by Consecutive Passages in Primary Hamster Kidney Tissue Culture

1.
National Institute for the Control of Pharmaceutical and Biological Products
2.
Beijing

Abstract

A dengue type 4 virus Ban18 strain, isolated from non-epidemic area in China, was chosen for attenuation by consecutive passages in primary hamster kidney tissue culture(PHKTC). During the course of passage from 20 to 70, the CPE in PHKTC became more and more apparent and a high virus titer up to 10~6-10~7 TCID_(50) per ml could be reached. Meanwhile the neurovirulence to suckling mice was gradually reduced from 10~6-10~7LD_(50)/ml for the parental virus to no death or only individual death of animal for th...
- Deugue type Ⅳ virus
- , Attenuation in primary hamster kindney cell culture

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study of Attenuation of Dengue Type 4 Virus by Consecutive Passages in Primary Hamster Kidney Tissue Culture

1. National Institute for the Control of Pharmaceutical and Biological Products
2. Beijing

Abstract: A dengue type 4 virus Ban18 strain, isolated from non-epidemic area in China, was chosen for attenuation by consecutive passages in primary hamster kidney tissue culture(PHKTC). During the course of passage from 20 to 70, the CPE in PHKTC became more and more apparent and a high virus titer up to 10~6-10~7 TCID_(50) per ml could be reached. Meanwhile the neurovirulence to suckling mice was gradually reduced from 10~6-10~7LD_(50)/ml for the parental virus to no death or only individual death of animal for th...