Cloning and ldentification of the Type-specific DNA Fragment of Herpes Simplex Virus Type 2

CHU Yong-Lie; FANG Yi-Lan; LIU Yan-Na; ZHOU Yu-Ling

PDF
Cite
Share

facebook

twitter

google

linkedin

June 1989

CHU Yong-Lie, FANG Yi-Lan, LIU Yan-Na and ZHOU Yu-Ling. Cloning and ldentification of the Type-specific DNA Fragment of Herpes Simplex Virus Type 2[J]. Virologica Sinica, 1989, 4(3).

Citation: CHU Yong-Lie, FANG Yi-Lan, LIU Yan-Na, ZHOU Yu-Ling. Cloning and ldentification of the Type-specific DNA Fragment of Herpes Simplex Virus Type 2 .VIROLOGICA SINICA, 1989, 4(3) : 245.

单纯疱疹病毒2型特异性DNA片段的克隆和鉴定

1.
西安医科大学微生物教研室
2.
西安医科大学微生物教研室西安

摘要

用核酸限制性内切酶BamHI对单纯疱疹病毒2型(HSV—2)的DNA进行酶解,回收位于基因组中的反向重复序列区的Bam HIG片段,然后将其克隆在载体质粒PUC 8的Bam HI切点上,进一步用核酸限制性内切酶Eco RI和KPNI对这一重组质粒联合酶解,移去EcoRI—KPNI小片段,经末端修饰后,将其连接得到新的重组质粒pRC102,它含有一小段HSV—2的DNA序列。以此质粒为探针,分别与HSV—1、HSV—2及细胞DNA进行斑点杂交;与HSV—1和HSV—2酶解后的DNA片段进行Southern转印系交。两组实验结果显示,pRC102质粒DNA只与HSV—2 DNA特异性杂交,其HSV—2的型特异性良好。
- HSV—2型特异性DNA片段
- , 基因克隆

Cloning and ldentification of the Type-specific DNA Fragment of Herpes Simplex Virus Type 2

1.
Dept. of Microbiology
2.
Xi-an Medical University

Abstract

The DNA of Herpes Simplex Virus type 2 (HSV-2)digested with restrictionenzyme BamHI and the BamHI G fragment was isolated, which locates in thereverse repeat sequence and spans the junction site of L and S component.Then the BamHI G was inserted on the BamHI cut-site of the vector plasmidpUC 8. In order to modify this recombinant plasmid, the EcoRI--KPN I fra-gment of the recombinant plasmid was removed by restriction enzyme digestionwith EcoRI and KPN 1. After modification of the ends and ligation of them,...
- Type-specific DNA fragment of HSV-2
- , Gene cloning

References
Proportional views

PDF

Article Metrics

Article views(3752) PDF downloads(868) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and ldentification of the Type-specific DNA Fragment of Herpes Simplex Virus Type 2

1. Dept. of Microbiology
2. Xi-an Medical University

Abstract: The DNA of Herpes Simplex Virus type 2 (HSV-2)digested with restrictionenzyme BamHI and the BamHI G fragment was isolated, which locates in thereverse repeat sequence and spans the junction site of L and S component.Then the BamHI G was inserted on the BamHI cut-site of the vector plasmidpUC 8. In order to modify this recombinant plasmid, the EcoRI--KPN I fra-gment of the recombinant plasmid was removed by restriction enzyme digestionwith EcoRI and KPN 1. After modification of the ends and ligation of them,...