Studies on the Isolation of Carnation Ringspot Virus by cDNA Hybridization Analysis and Serology

AN De-Rong; WEI Ning-Sheng; R.Koeni

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August 1989

AN De-Rong, WEI Ning-Sheng and R.Koeni. Studies on the Isolation of Carnation Ringspot Virus by cDNA Hybridization Analysis and Serology[J]. Virologica Sinica, 1989, 4(4).

Citation: AN De-Rong, WEI Ning-Sheng, R.Koeni. Studies on the Isolation of Carnation Ringspot Virus by cDNA Hybridization Analysis and Serology .VIROLOGICA SINICA, 1989, 4(4) : 408.

互补DNA杂交分析和血清学方法对香石竹环斑病毒分离物的研究

1.
西北农业大学植保系
2.
联帮德国生物研究中心病毒所杨陵

摘要

在联邦德国奥卡河中分离到一种病毒分离物(W),通过鉴别寄主反应的测定,病毒粒子的电子显微镜观察,cDNA斑点杂交和血清学的研究,鉴定出这一病毒分离物为香石竹环斑病毒。其外壳蛋白亚基的分子量为3.8×10~4。cDNA吸印转移杂交分析表明,香石竹环斑病毒的两个RNA组份(RNA~1、RNA_2)之间没有核苷酸序列同源性。RNA_1和RNA_2分别由3.7和1.5仟碱基组成。
- 香石竹环斑病毒
- , 互补DNA
- , 血清学
- , RNA序列同源性

Studies on the Isolation of Carnation Ringspot Virus by cDNA Hybridization Analysis and Serology

1.
Dept.of Plant Protection
2.
Northwestern Agricultural University

Abstract

A virus isolate (w) has been obtained from the Oker river in W.Germany. The isolate was identified as Carnation ringspot virus (CarRSV) by means of inoculation on the test plant, electron microscopy, dot blot hybridization and serology.The molecular weight of the coat protein is 3.8×10~4. Northern blot analysis revealed that the two RNA species of the virus which consisted of c. 3.7 and 1.5kb, respectively, but without base sequence homology.
- CarRSV
- , cDNA hybridization
- , RNA sequence homology

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on the Isolation of Carnation Ringspot Virus by cDNA Hybridization Analysis and Serology

1. Dept.of Plant Protection
2. Northwestern Agricultural University

Abstract: A virus isolate (w) has been obtained from the Oker river in W.Germany. The isolate was identified as Carnation ringspot virus (CarRSV) by means of inoculation on the test plant, electron microscopy, dot blot hybridization and serology.The molecular weight of the coat protein is 3.8×10~4. Northern blot analysis revealed that the two RNA species of the virus which consisted of c. 3.7 and 1.5kb, respectively, but without base sequence homology.