XU Ren-Lin and YI Qiong-Hua. Effects of Temperature on ToMV Multiplication and Changes of Soluble Leaf Proteins in Tomato[J]. Virologica Sinica, 1989, 4(4).
Citation:
XU Ren-Lin, YI Qiong-Hua.
Effects of Temperature on ToMV Multiplication and Changes of Soluble Leaf Proteins in Tomato .VIROLOGICA SINICA, 1989, 4(4)
: 415.
温度对番茄花叶病毒(TOMV)增殖及番茄叶片可溶性蛋白变化的影响
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1.
江苏省农科院遗传生理所
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2.
江苏省农科院遗传生理所 南京
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摘要
局部枯斑法测定结果表明,番茄GCR—267品系叶片内ToMV的含量仅为番茄GCR-26品系叶片内病毒含量的1/40—1/50;GCR-267品系含有的Tm-2~(?)基因对ToMV增殖的抑制作用不受温度变化的影响;而ToMV在GCR-26体内的增殖却依赖于环境温度,高温对它有部分的抑制作用。用非变性聚丙烯酰胺凝胶电泳和SDS—聚丙烯酰胺凝胶电泳分析上述两个品系在常温和高温下接种ToMV后其叶片可溶性蛋白的变化,结果发现在GCR-26品系中,ToMV的增殖与寄主体内新产生的14.2KD蛋白呈正相关,而在GCR—267品系中未检测到这种蛋白。我们推测14.2KD蛋白可能是一种能参与或促进ToMV增殖过程的温度敏感因子,称之为番茄“S”蛋白。
Effects of Temperature on ToMV Multiplication and Changes of Soluble Leaf Proteins in Tomato
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Abstract
ToMV concentrations in virus inoculated tomato plants were analyzed by local lesion test. The results showed that virus concentrations in resistan-t line GCR-267 was 1/40—1/50 that in susceptible line GCR-26. ToMV multiplication was strongly inhibited by gene Tm-2~a, which was not influenced by temperature. ToMV multiplication in GCR-26 was dependent on temperature, the higher the temperatature, the less the virus concentration. The changes of the soluble leaf proteins of tomato plants grown at constant or ...
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