Development of Method for the Detection of Enteroviruses from Clams

MA Ju-Fang; JIANG Hui-Hui; HU Shan-Lian; YAN Hui-Qin; LI Jie

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February 1990

MA Ju-Fang, JIANG Hui-Hui, HU Shan-Lian, YAN Hui-Qin and LI Jie. Development of Method for the Detection of Enteroviruses from Clams[J]. Virologica Sinica, 1990, 5(1).

Citation: MA Ju-Fang, JIANG Hui-Hui, HU Shan-Lian, YAN Hui-Qin, LI Jie. Development of Method for the Detection of Enteroviruses from Clams .VIROLOGICA SINICA, 1990, 5(1) : 41.

毛蚶中肠道病毒检测方法的建立

1.
上海医科大学公共卫生学院
2.
上海医科大学公共卫生学院上海

摘要

本文建立了检测毛蚶中肠道病毒的方法。病毒在pH4.5时吸附于毛蚶组织,离心后用pH9.5甘氨酸盐水将病毒从毛蚶组织中洗脱出来,并用0.0005mol/L三氯化铝代替Johnson法中的Cat—Floc,达到去除细胞毒性物质的目的。经过再一次离心,利用牛肉浸膏在pH3.5—4.0时凝聚并吸附病毒的特性使洗脱液中的病毒得到浓缩。离心后沉淀溶解于0.1mol/L磷酸氢二钠溶液。用本法提取毛蚶中脊髓灰质炎病毒,当病毒含量为每克组织3.5×10~5至35PFU时,平均回收率为71.2％。
- 毛蚶
- , 脊髓灰质炎病毒
- , 回收率

Development of Method for the Detection of Enteroviruses from Clams

1.
School of Public Health
2.
Shanghai Medical University

Abstract

A procedure for concentrating and recovering enteroviruses from clams (Arca( Anadara)subcrenata Lischke)has been developed and evaluated. Viruses were efficiently adsorbed to homogenized clam tissue by adjusting the homogenate to pH4.5. After low-speed centrifugation, the viruses were eluted from the sediment with pH 9.5 0.05mol/L glycine-saline. Instead of using Cat-Floc in Johnson s method, AlCl_3 was used to remove cytotoxity from clam tissue homogenates. After low-speed centrifugation, viruses were conc...
- Clam （Arca （Anadara） subcrerata Lischke） Poliovirus recovery of virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Development of Method for the Detection of Enteroviruses from Clams

1. School of Public Health
2. Shanghai Medical University

Abstract: A procedure for concentrating and recovering enteroviruses from clams (Arca( Anadara)subcrenata Lischke)has been developed and evaluated. Viruses were efficiently adsorbed to homogenized clam tissue by adjusting the homogenate to pH4.5. After low-speed centrifugation, the viruses were eluted from the sediment with pH 9.5 0.05mol/L glycine-saline. Instead of using Cat-Floc in Johnson s method, AlCl_3 was used to remove cytotoxity from clam tissue homogenates. After low-speed centrifugation, viruses were conc...