Detection of HIV Antibody by Bridge-linked Enzyme Labelling Technique

WANG Zhe; ZENG Yi

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June 1990

WANG Zhe and ZENG Yi. Detection of HIV Antibody by Bridge-linked Enzyme Labelling Technique[J]. Virologica Sinica, 1990, 5(3).

Citation: WANG Zhe, ZENG Yi. Detection of HIV Antibody by Bridge-linked Enzyme Labelling Technique .VIROLOGICA SINICA, 1990, 5(3) : 291.

应用桥联酶标技术检测艾滋病毒抗体

王哲 ,
曾毅

1.
中国预防医学科学院艾滋病研究和检测中心
2.
中国预防医学科学院艾滋病研究和检测中心北京

摘要

将APAAP桥联酶标技术用于HIV抗体检测,其特异性和重复性与免疫荧光技术相同,敏感性高于后者。APAAP法易辨认掌握,不需特殊仪器,适用于基层和临床使用。
- 艾滋病病毒
- , APAAP桥联酶标技术
- , 抗体检测

Detection of HIV Antibody by Bridge-linked Enzyme Labelling Technique

WANG Zhe ,
ZENG Yi

1.
AIDS Research and Detection Centre
2.
Chinese Academy Preventive Medicine

Abstract

APAAP bridge-linked enzyme labelling method was established for detection of HIV antibody. Compared with immunofluorescence test (IF), these two methods had the same specificity and reproducibility, but APAAP is more sensitive for detection of HIV antibody than IF test. The technique is simple and convenient, and only requires the ordinary microscope, so it can be applied in HIV screening laboratories and clinic.
- HIV APAAP bridge-linked enzyme labelling test Antibody detection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of HIV Antibody by Bridge-linked Enzyme Labelling Technique

WANG Zhe ,
ZENG Yi

1. AIDS Research and Detection Centre
2. Chinese Academy Preventive Medicine

Abstract: APAAP bridge-linked enzyme labelling method was established for detection of HIV antibody. Compared with immunofluorescence test (IF), these two methods had the same specificity and reproducibility, but APAAP is more sensitive for detection of HIV antibody than IF test. The technique is simple and convenient, and only requires the ordinary microscope, so it can be applied in HIV screening laboratories and clinic.