YANG Tie-Qiu, LI Zhi-Hong, YANG Beng, LIU Wei-Qian, DIAO Wen-Xian, LI De-Xin and HANG Chang-Shou. Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization[J]. Virologica Sinica, 1991, 6(4).
Citation:
YANG Tie-Qiu, LI Zhi-Hong, YANG Beng, LIU Wei-Qian, DIAO Wen-Xian, LI De-Xin, HANG Chang-Shou.
Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization .VIROLOGICA SINICA, 1991, 6(4)
: 320.
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摘要
为寻找一种用于检测流行性出血热病毒的分子杂交方法,以生物素-7-dATP标记流行性出血热病毒(EHFV)R_(22)株M片段的cDNAR_3克隆作探针,与人源性的EHFVH-114、H-435株RNA基因组进行斑点杂交,得到阳性结果,可检出5pg的cDNA或RNA。此探针与疱疹病毒DNA不出现杂交信号。以上结果说明这种标记探针具有EHFV特异性,可以扩大应用范围,结果还表明动物源性和人源性EHFV均具有共同的保守核苷酸序列。
Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization
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1.
Virus Research Institute
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2.
Hubei Medical College
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3.
Wuhan
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Abstract
Dot hybridization assay was developed to detect epidemic hemorrhagic fever virus(EHFV)RNA genome using probe which biotin-7-dATP labeled R-3 cDNA clone of M fragement of EHFV R-22 strain. This probe was used to detect RNA genome of the human original EHFV H-114 and H-435 strains in cell cultures. The positive hybridization signal was obtained from cells infected EHFV H-114 and H-435 strains, 5 pg cDNA or RNA could be obtained This probe do not react with DNA of cells infected herpes simplex virus and cytome...
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References
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Proportional views
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