Analysis of peptides of HBsAg and the Epitopes of "a" Determinant by Monoclonal Antihody

WANG You-Chun; LI He-Min; HU Zong-Han

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February 1992

WANG You-Chun, LI He-Min and HU Zong-Han. Analysis of peptides of HBsAg and the Epitopes of 'a' Determinant by Monoclonal Antihody[J]. Virologica Sinica, 1992, 7(1).

Citation: WANG You-Chun, LI He-Min, HU Zong-Han. Analysis of peptides of HBsAg and the Epitopes of "a" Determinant by Monoclonal Antihody .VIROLOGICA SINICA, 1992, 7(1) : 48.

用单克隆抗体分析HBsAg的多肽及a决定簇的表位

1.
中国药品生物制品检定所
2.
中国药品生物制品检定所北京
3.
100050

摘要

通过Western-Blot发现,多克隆抗-HBs抗体及单克隆抗体B_(13),A_1均识别24kd及27kd两条多肽带,而单克隆抗体B_6,B_9及B_(12)只识别24kd,不识别27kd的多肽带。用这几株单克隆抗体对不同来源HBsAg a决定簇的表位密度进行了研究,结果表明血源、重组CHO细胞表达及重组痘苗病毒表达的HBsAg与重组酵母表达的HBsAg在结合单克隆抗体A_1、B_6及B_9的密度上有数量的差别,并且有统计学意义。
- HBsAg
- , a决定簇
- , 表位
- , 单克隆抗体

Analysis of peptides of HBsAg and the Epitopes of "a" Determinant by Monoclonal Antihody

1.
National Institute for the Control of Pharmaceutical and Biological Products
2.
Beijing 100050

Abstract

Five selected McAbs were tested for their binding to HBsAg polypeptidecomposition by using Western Blot. McAb B_6, B_9 and B_(12) combined polypeptideP24 of HBsAg, while McAb A_1 and B_(13) combined both polypeptide p24 andP27 of HBsAg. Using the five McAbs, the epitope density of "a" determinantof HBsAg derived from different sources was also studied with the second an-tibody method. The result showed that there was similar density of epitopesagainst five McAbs on plasma-derived HBsAg (PD HBsAg), CHO cell-...
- HBsAg a determinant
- , Epitope
- , Monoclonal antibody

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of peptides of HBsAg and the Epitopes of "a" Determinant by Monoclonal Antihody

1. National Institute for the Control of Pharmaceutical and Biological Products
2. Beijing 100050

Abstract: Five selected McAbs were tested for their binding to HBsAg polypeptidecomposition by using Western Blot. McAb B_6, B_9 and B_(12) combined polypeptideP24 of HBsAg, while McAb A_1 and B_(13) combined both polypeptide p24 andP27 of HBsAg. Using the five McAbs, the epitope density of "a" determinantof HBsAg derived from different sources was also studied with the second an-tibody method. The result showed that there was similar density of epitopesagainst five McAbs on plasma-derived HBsAg (PD HBsAg), CHO cell-...