Use of Polymerase Chain Reaction for Detection of the Seguence Related to HPV16 E6/E7 in Cervical Cancers

LIU Xua-Feng; YANG Ping; WU Xin-Xing; DING Xiao-Hua; LIU Wei-Qian; ZHAO Wen-Xian

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February 1992

LIU Xua-Feng, YANG Ping, WU Xin-Xing, DING Xiao-Hua, LIU Wei-Qian and ZHAO Wen-Xian. Use of Polymerase Chain Reaction for Detection of the Seguence Related to HPV16 E6/E7 in Cervical Cancers[J]. Virologica Sinica, 1992, 7(1).

Citation: LIU Xua-Feng, YANG Ping, WU Xin-Xing, DING Xiao-Hua, LIU Wei-Qian, ZHAO Wen-Xian. Use of Polymerase Chain Reaction for Detection of the Seguence Related to HPV16 E6/E7 in Cervical Cancers .VIROLOGICA SINICA, 1992, 7(1) : 59.

聚合酶链反应检测宫颈癌中HPV16E_6/E_7相关序列

1.
湖北医学院病毒所
2.
湖北医学院病毒所武汉 430071 湖北医学院生化教研室

摘要

本文报告了采用聚合酶链反应(即PCR技术)检测人宫颈癌中人乳头瘤病毒16型(HPV16)的E_6/E_7相关序列。在正链引物(SP)和反链引物(ASP)的诱导下,以二步温控法聚合酶链反应对34例人宫颈癌组织DNA进行检测,发现宫颈癌中HPV16E_6/E_7相关序列存在的阳性率达67.6％(23/34)。本研究结果提示:HPV16可能在宫颈癌的发生发展过程中起重要作用,采用PCR技术选择性地检测HPV16的转化基因(E6、E7)对深入探讨宫颈癌发生的机理、早期发现宫颈癌患者等有重要意义。
- 人乳头瘤病毒
- , 转化基因
- , 宫颈癌
- , 聚合酶链反应

Use of Polymerase Chain Reaction for Detection of the Seguence Related to HPV16 E6/E7 in Cervical Cancers

1.
Virus Research Institute
2.
Hubei Medical College

Abstract

Detection of the sequence related to E6/E7 of human papillomavirus type16 in Chinese cervical cancers using polymerase chain reaction (PCR) has beenreported in this paper. We have used the two-step protocol PCR to detectDNA sequence related to HPV16 E6/E7 in 34 cervical cancer tissues, by primingwith SP and ASP, the positive percentage of HPV 16 E6/E7 in cervical cancerswas 67.6% (23/34). This result showed that HPV 16 may play an importantrole in development and progression of cervical cancers, and u...
- Human Papillomavirus
- , Transforming genes
- , Cervical cancers
- , Polymerase chain reaction

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Use of Polymerase Chain Reaction for Detection of the Seguence Related to HPV16 E6/E7 in Cervical Cancers

1. Virus Research Institute
2. Hubei Medical College

Abstract: Detection of the sequence related to E6/E7 of human papillomavirus type16 in Chinese cervical cancers using polymerase chain reaction (PCR) has beenreported in this paper. We have used the two-step protocol PCR to detectDNA sequence related to HPV16 E6/E7 in 34 cervical cancer tissues, by primingwith SP and ASP, the positive percentage of HPV 16 E6/E7 in cervical cancerswas 67.6% (23/34). This result showed that HPV 16 may play an importantrole in development and progression of cervical cancers, and u...