Localization and Cloning of the Polyhedrin Gene of Buzura suppressaria Nuclear Polyhedrosis Virus

LIU Ming-Fu; HU Zhi-Hong; JIN Feng; LI Meng-Jin; XIE Tian-En

PDF
Cite
Share

facebook

twitter

google

linkedin

February 1992

LIU Ming-Fu, HU Zhi-Hong, JIN Feng, LI Meng-Jin and XIE Tian-En. Localization and Cloning of the Polyhedrin Gene of Buzura suppressaria Nuclear Polyhedrosis Virus[J]. Virologica Sinica, 1992, 7(1).

Citation: LIU Ming-Fu, HU Zhi-Hong, JIN Feng, LI Meng-Jin, XIE Tian-En. Localization and Cloning of the Polyhedrin Gene of Buzura suppressaria Nuclear Polyhedrosis Virus .VIROLOGICA SINICA, 1992, 7(1) : 80.

油桐尺蠖核多角体病毒多角体蛋白基因定位与克隆

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

以[~(32)P]-dATP标记含AcNPV DNA的EcoRI-I片段的重组质粒为探针,在35℃条件下对油桐尺蠖核多角体病毒(BsNPV)多角体蛋白基因进行了定位,将其分别定位在BamH Ⅰ-A,Bgl Ⅰ-A,Bgl Ⅱ-F,EocR Ⅰ-R,Hind Ⅲ-A,Kpn Ⅰ-Ⅰ,Pst Ⅰ-D,Xba Ⅰ-A(或B),Xho Ⅰ-F和G片段上,并以M13mp18为载体,克隆了Kpn Ⅰ-Ⅰ片段。
- 油桐尺蠖核多角体病毒
- , 多角体蛋白基因
- , 分子杂交
- , 同源性

Localization and Cloning of the Polyhedrin Gene of Buzura suppressaria Nuclear Polyhedrosis Virus

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA EcoRI-I as the probe. For sequencing the polyhedringene, the KpnI-I fragment was cloned into M13mp18 vector.
- Buzura suppressaria nuclear polyhedrosis virus（BsNPV）
- , Polyhedrin gene Hybridization

References
Proportional views

PDF

Article Metrics

Article views(3726) PDF downloads(1006) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Localization and Cloning of the Polyhedrin Gene of Buzura suppressaria Nuclear Polyhedrosis Virus

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA EcoRI-I as the probe. For sequencing the polyhedringene, the KpnI-I fragment was cloned into M13mp18 vector.