A Single--Stranded Virus Isolated from Shiitake Mushroom Lentinus edodes (Berk.) Sing.

CHEN Xue-Ren; CHEN Ju-Ying; CHEN Zuo-Yi; GONG Jie-Xun; CHEN Ming-Jie; PAN Ying-Cha; WANG Zhao-Yue; FANG Bing-Chu; WANG Ming-Qi

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February 1992

CHEN Xue-Ren, CHEN Ju-Ying, CHEN Zuo-Yi, GONG Jie-Xun, CHEN Ming-Jie, PAN Ying-Cha, WANG Zhao-Yue, FANG Bing-Chu and WANG Ming-Qi. A Single--Stranded Virus Isolated from Shiitake Mushroom Lentinus edodes (Berk.) Sing.[J]. Virologica Sinica, 1992, 7(1).

Citation: CHEN Xue-Ren, CHEN Ju-Ying, CHEN Zuo-Yi, GONG Jie-Xun, CHEN Ming-Jie, PAN Ying-Cha, WANG Zhao-Yue, FANG Bing-Chu, WANG Ming-Qi. A Single--Stranded Virus Isolated from Shiitake Mushroom Lentinus edodes (Berk.) Sing. .VIROLOGICA SINICA, 1992, 7(1) : 99.

一种含有单链RNA的香菇球状病毒

1.
中国科学院上海生物化学研究所
2.
上海农业科学院食用菌研究所
3.
复旦大学生命科学院微生物及微生物工程系上海

摘要

从生长不正常的香菇(Lentinus edodes(Berk.)Sing)菌株中分离到一种等轴对称含单链RNA的病毒颗粒。病毒颗粒在电镜下直径为33～34nm,在SDS-聚丙烯酰胺凝胶电泳中病毒外壳蛋白分子量为22000道尔顿。病毒核酸径DNase1和SI酶解试验及热变性紫外吸收曲线试验证明为单链RNA,在1.5％的琼脂糖凝胶电泳中,病毒核酸呈现一条带,分子量为2.3810~6道尔顿。
- 香菇病毒
- , 病毒形态
- , 理化性质

A Single--Stranded Virus Isolated from Shiitake Mushroom Lentinus edodes (Berk.) Sing.

1.
Shanghai Institute Biochemistry
2.
Academia Sinica
3.
Shanghai 200031 Edible Fungi Research Institute

Abstract

A isometric virus-like particles(VLPs), which are approximately 34nm indiameter containing ss-RNA, have been purified from abnormal mycelial ofshiitake mushroom Leutinus edodes (Berk.) Sing. The SDS-polyacrylamide gelelectrophoresis demonstrated that the virions contain a single capsid polypeptideband with molecular weight about 22000 dalton. The nucleic acid extractedfrom purified preparations of 34nm VLPs showed only one band with mole-cular weight approximately 2.3810~6 dalton on the 1.5% agarose gel. T...
- Shiitake mushroom virus （Lentinus edodes virus）
- , Size and morphology of virions
- , Properties

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A Single--Stranded Virus Isolated from Shiitake Mushroom Lentinus edodes (Berk.) Sing.

1. Shanghai Institute Biochemistry
2. Academia Sinica
3. Shanghai 200031 Edible Fungi Research Institute

Abstract: A isometric virus-like particles(VLPs), which are approximately 34nm indiameter containing ss-RNA, have been purified from abnormal mycelial ofshiitake mushroom Leutinus edodes (Berk.) Sing. The SDS-polyacrylamide gelelectrophoresis demonstrated that the virions contain a single capsid polypeptideband with molecular weight about 22000 dalton. The nucleic acid extractedfrom purified preparations of 34nm VLPs showed only one band with mole-cular weight approximately 2.3810~6 dalton on the 1.5% agarose gel. T...