Neutralizing Antibody Responses in Human Immunized with M. Gerbil Kidney Tissue Culture Inactivated HFRS Vaccine Assessed by Two Methods

SHU Yong-Xin; LIU Wen-Xue; NIE Zi-Lin

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April 1992

SHU Yong-Xin, LIU Wen-Xue and NIE Zi-Lin. Neutralizing Antibody Responses in Human Immunized with M. Gerbil Kidney Tissue Culture Inactivated HFRS Vaccine Assessed by Two Methods[J]. Virologica Sinica, 1992, 7(2).

Citation: SHU Yong-Xin, LIU Wen-Xue, NIE Zi-Lin. Neutralizing Antibody Responses in Human Immunized with M. Gerbil Kidney Tissue Culture Inactivated HFRS Vaccine Assessed by Two Methods .VIROLOGICA SINICA, 1992, 7(2) : 176.

两种方法检测接种流行性出血热沙鼠肾细胞灭活疫苗后人体的中和抗体

1.
中国药品生物制品检定所
2.
中国药品生物制品检定所北京

摘要

本文应用空斑减少中和试验(PRNT)和细胞病变中和试验(cPENT)两种方法对出血热沙鼠肾细胞灭活疫苗扩大人体免疫后的血清进行中和抗体水平检测。根据两种方法对总计74人份的免疫后血清检测比较结果,两种方法检测的抗体阳转率和抗体水平(GMT)。CPENT法均高于PRNT法,经统计学处理均有显著性差异。不同免疫组的中和抗体水平比较结果,注射三针的阳转率(n=10,100％)高于两针组(n=10,20—30％);接种加氢氧化铝佐剂疫苗(n=13)较接种不加佐剂的两种疫苗(n=26)的抗体水平高,阳转率为92％—100％GMT为22—69;皮下途径(n=15)和肌肉途径(n=13)注射无明显差别,阳转率分别为87—93％和92—100％,GMT分别为29—46和22—61。以上结果进一步肯定沙鼠肾细胞疫苗的人体免疫性
- 流行性出血热病毒
- , 沙鼠肾细胞灭活疫苗
- , 扩大人体免疫
- , 空斑减少
- , 中和试验
- , 细胞病变中相试验

Neutralizing Antibody Responses in Human Immunized with M. Gerbil Kidney Tissue Culture Inactivated HFRS Vaccine Assessed by Two Methods

1.
National Institute for the Contrl of Pharmacenlical & Biological Products
2.
Beijing 100050

Abstract

In this study, expanded human trial with M, Gerbil kidney tissue culture inactivated HFRS vaccine was carried out and neutralizing antibody response was assessed by plaque reduction neutralization(PRNT) and CPE neutralization(CPENT) methods. According to the data of all 74 person immune sera assayed by the two methods, the rates of seroconversion and GMT tesed by CPENT were significantly higher than that by PRNT. Several vaccinating groups were studied and the neutralizing antibody levels were as follows: v...
- Homorrhagic Fever with Renal Syndrome M. Gerbil tissue culture inaetivated vaccine Human expounded trial PRNT CPENT

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Neutralizing Antibody Responses in Human Immunized with M. Gerbil Kidney Tissue Culture Inactivated HFRS Vaccine Assessed by Two Methods

1. National Institute for the Contrl of Pharmacenlical & Biological Products
2. Beijing 100050

Abstract: In this study, expanded human trial with M, Gerbil kidney tissue culture inactivated HFRS vaccine was carried out and neutralizing antibody response was assessed by plaque reduction neutralization(PRNT) and CPE neutralization(CPENT) methods. According to the data of all 74 person immune sera assayed by the two methods, the rates of seroconversion and GMT tesed by CPENT were significantly higher than that by PRNT. Several vaccinating groups were studied and the neutralizing antibody levels were as follows: v...