Induction and Enhancement of CPE in HFRS Viruses Infected Vero-E6 Cells by HEPES

NIE Zi-Lin; YU Yong-Xin

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April 1993

NIE Zi-Lin and YU Yong-Xin. Induction and Enhancement of CPE in HFRS Viruses Infected Vero-E6 Cells by HEPES[J]. Virologica Sinica, 1993, 8(2).

Citation: NIE Zi-Lin, YU Yong-Xin. Induction and Enhancement of CPE in HFRS Viruses Infected Vero-E6 Cells by HEPES .VIROLOGICA SINICA, 1993, 8(2) : 132.

HEPES诱导增强HFRS病毒对Vero-E_6细胞的致细胞病变作用

聂子林 ,
俞永新

1.
中国药品生物制品检定所
2.
中国药品生物制品检定所北京

摘要

本文报道了HEPES诱导增强HFRS病毒对Vero-E_6细胞的致细胞病变作用。病毒悬浮接种E_6细胞,置37℃培养2—8天后在培养液中加适量HEPES,24小时内即见明显的细胞病变出现。细胞病变的特点是感染细胞相互融合形成多核巨细胞或网状结构,有时则出现泡沫样的空泡结构。HEPES诱导增强的细胞病变能被特异抗HFRS病毒免疫血清和单克隆抗体等中和抑制。HEPES的这一特性对建立简便的HFRS病毒实验方法等具有较大的意义。
- HEPES HFRS病毒
- , 致细胞病变作用
- , Vero—E_6细胞

Induction and Enhancement of CPE in HFRS Viruses Infected Vero-E6 Cells by HEPES

NIE Zi-Lin ,
YU Yong-Xin

1.
National Institute for the Control of Pharmaceutical and Biological Products
2.
Beijing 100050

Abstract

We have found that HEPES could induce and enhance the CPE caused by HFRS viruses in cultured Vero-E_6 cells. The infected cells were treated with 10-100mmol/L HEPES at 2—8 days post inoculation, several hours later, polykaryocytoses, networks and vacuoles appeared in the cell cultures. It could be seen under microscope without staining. On the contrast, no CPE was found in the untreated infective Vero-E_6 control cells or treated uninfected control cells The Vero-E_6 cells infected with 76-118, L_(99), HB_(...
- HFRS viruses Cytopathic effect Vero-E_6 cells

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Induction and Enhancement of CPE in HFRS Viruses Infected Vero-E6 Cells by HEPES

NIE Zi-Lin ,
YU Yong-Xin

1. National Institute for the Control of Pharmaceutical and Biological Products
2. Beijing 100050

Abstract: We have found that HEPES could induce and enhance the CPE caused by HFRS viruses in cultured Vero-E_6 cells. The infected cells were treated with 10-100mmol/L HEPES at 2—8 days post inoculation, several hours later, polykaryocytoses, networks and vacuoles appeared in the cell cultures. It could be seen under microscope without staining. On the contrast, no CPE was found in the untreated infective Vero-E_6 control cells or treated uninfected control cells The Vero-E_6 cells infected with 76-118, L_(99), HB_(...