Detection of Epstein-Barr Virus Gene in Biopsied Tissue and Nasopharyngeal Epithelial Cells by Polymerase Chain Reaction

HONG Hui-Min; CHEN Jun; WU Qiu-Liang; JIAN Shao-Wen; LI Man-Zhi; TUN Yin-Tang

PDF
Cite
Share

facebook

twitter

google

linkedin

April 1993

HONG Hui-Min, CHEN Jun, WU Qiu-Liang, JIAN Shao-Wen, LI Man-Zhi and TUN Yin-Tang. Detection of Epstein-Barr Virus Gene in Biopsied Tissue and Nasopharyngeal Epithelial Cells by Polymerase Chain Reaction[J]. Virologica Sinica, 1993, 8(2).

Citation: HONG Hui-Min, CHEN Jun, WU Qiu-Liang, JIAN Shao-Wen, LI Man-Zhi, TUN Yin-Tang. Detection of Epstein-Barr Virus Gene in Biopsied Tissue and Nasopharyngeal Epithelial Cells by Polymerase Chain Reaction .VIROLOGICA SINICA, 1993, 8(2) : 142.

用PCR技术检测活检组织和鼻咽上皮细胞内EB病毒基因

1.
中山医科大学肿瘤研究所
2.
中山医科大学肿瘤医院病理科
3.
中山医科大学肿瘤研究所广州

摘要

选用Epstin-Barr病毒(EBV)基因组内部重复序列1(IR1)片断作为多聚酶链反应(Polymerase Chain Reaction,PCR)扩增引物,用于检测了31例不同病例活检组织和4例新鲜鼻咽组织经体外培养6周以上的新生上皮细胞内EBV基因,其中检出EBVDNA:高分化鼻咽癌5/5,低分化鼻咽癌4/4,何杰金氏病5/5,非何杰金氏病0/2,头颈其他肿瘤1/6,鼻咽慢性炎症0/5,正常鼻咽组织0/4;新生上皮细胞DNA抽提物;低分化鼻咽癌2/2,炎症0/1,正常人胚鼻咽上皮0/1;携带EBV基因组细胞系(Raji,B_(95-8)各1)2/2,致淋巴细胞转化之B_(95-8)病毒为10~(-4),PCR检测10~(-4)～10~(-6)均阳性,10~(-7)未检出。结果表明EBV与鼻咽癌与何杰金氏病有关,常规石蜡包埋切片仅8μm×0.1mm~2,贮存时间至三年仍可用于PCR检测EBV DNA,证实PCR是一种快速、灵敏和特异测捡EBV基因组的方法,可作为肿瘤和疚病病毒病因回顾性调直研究的有力手段。
- EB病毒
- , 鼻咽癌
- , 何杰金氏病
- , 多聚酶链反应

Detection of Epstein-Barr Virus Gene in Biopsied Tissue and Nasopharyngeal Epithelial Cells by Polymerase Chain Reaction

1.
Cancer Institute
2.
Sun Yat-Sen University of Medical Sciences
3.
Gnangzhou

Abstract

The amplifying target sequence for Polymerase Chain Reaction(PCR) was chosen within the Internal Repeat Ⅰ (IR Ⅰ) of EBV genome. We used PCR of the primers to detect 31 biopsied specimens of different disease and 4 nasopharyngeal epithelial cultures. EBV genomes were detected in differentiated nasopharyngeal carcinoma(NPC)biopsies 5/5, poorly differentiated NPC 4/4, Hodgkin s Disease (HD) 3/5, non-Hodgkin s Disease (NHD) 0/2. Other head and neck tumors 1/6, chronic inflammatory nasopharyngeal 0/5, normal nas...
- Epstein-Barr virus Nasopharyngeal carcinoma Hodgkin s disease Polymerase chain reaction

References
Proportional views

PDF

Article Metrics

Article views(3709) PDF downloads(834) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Epstein-Barr Virus Gene in Biopsied Tissue and Nasopharyngeal Epithelial Cells by Polymerase Chain Reaction

1. Cancer Institute
2. Sun Yat-Sen University of Medical Sciences
3. Gnangzhou

Abstract: The amplifying target sequence for Polymerase Chain Reaction(PCR) was chosen within the Internal Repeat Ⅰ (IR Ⅰ) of EBV genome. We used PCR of the primers to detect 31 biopsied specimens of different disease and 4 nasopharyngeal epithelial cultures. EBV genomes were detected in differentiated nasopharyngeal carcinoma(NPC)biopsies 5/5, poorly differentiated NPC 4/4, Hodgkin s Disease (HD) 3/5, non-Hodgkin s Disease (NHD) 0/2. Other head and neck tumors 1/6, chronic inflammatory nasopharyngeal 0/5, normal nas...