The Inhibitory Effects of Quinolones on Hepatitis B Virus in Vitro

FAN Chao; FU Xi-Xian; ZHANG Guo-Qiang; ZHANG Ai-Lin

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April 1994

FAN Chao, FU Xi-Xian, ZHANG Guo-Qiang and ZHANG Ai-Lin. The Inhibitory Effects of Quinolones on Hepatitis B Virus in Vitro[J]. Virologica Sinica, 1994, 9(2): 113-118.

Citation: FAN Chao, FU Xi-Xian, ZHANG Guo-Qiang, ZHANG Ai-Lin. The Inhibitory Effects of Quinolones on Hepatitis B Virus in Vitro .VIROLOGICA SINICA, 1994, 9(2) : 113-118.

喹诺酮类药物抗乙型肝炎病毒体外实验研究

1.
北京医科大学第一医院传染病教研室，北京100034

摘要

本文以２．２．１５细胞株为模型，以ＨＢｓＡｇ、ＨＢｅＡｇ、ＨＢＶＤＮＡ、细胞存活率为观察指标，综合评价了喹诺酮类药物吡哌酸（ＰｉｐｅｍｉｄｉｃＡｃｉｄ）、氟哌酸（Ｎｏｒｆｌｏｘａｃｉｎ）、环丙氟哌酸（Ｃｉｐｒｏｆｌｏｓｘａｃｉｎ）、氟嗪酸（Ｏｆｌｏｘａｃｉｎ）体外抗ＨＢＶ效果。结果表明：吡哌酸、氟哌酸、环丙氟哌酸、氟嗪酸对ＨＢｓＡｇ、ＨＢｅＡｇ５０％抑制浓度（ＩＤ＿（５０））分别为１１ｇ／ｍｌ、６４ｇ／ｍｌ、９３ｇ／ｍｌ、１０５ｇ／ｍｌ和１９９ｇ／ｍｌ、１１１ｇ／ｍｌ、２４ｇ／ｍｌ、２１７ｇ／ｍｌ，细胞存活率为５０％时的药物浓度（ＣＤ＿（５０））分别为２１９ｇ／ｍｌ、９０ｇ／ｍｌ、１８１ｇ／ｍｌ、１６９ｇ／ｍｌ，在所选定的用药浓度范围内不同程度抑制培养上清液及细胞内ＨＢＶＤＮＡ及其复制中间体的产生。尤其对超螺旋结构ＤＮＡ（ｓｃＤＮＡ）有不完全抑制作用。
- HBVDNA转染细胞株
- , Southern吸印杂交
- , 喹诺酮类药物

The Inhibitory Effects of Quinolones on Hepatitis B Virus in Vitro

1.
The farst teachang Hospital ,Beijing

Abstract

uinolones were studied for their ability to inhibit hepatitis B virus DNA replicatiun,HBsAg andHBeAg exporssion in a HBV-transfeeted eell line(2.2.15 cell).The survival cell rate was determinedby MTT assay.The result shows that ID50(the drug concentration that inhibits HBsAg or HBeAg secretion by 50%) were 110g/ml and 199g/ml with Pipemidic Acid,64g/ml and 111g/ml with Norfloxacin,93g/ml and 210g/ml with Ciprofloxacin,105g/ml and 217g/ml with Ofloxacin.CD_50(the drug concentration that reduces cell growth by 50%) were 219g/ml,90g/ml,181g/ml,160g/ml,reepectively.In the range of drug concentration selected,they partially or completelybleeked the production of HBV particles and its replicative interniediates,especially the seneration of suporcoiled viral DNA((sc DNA).
- HBV DNA
- , Transfected cell line
- , Southern blot hybridimtion

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Inhibitory Effects of Quinolones on Hepatitis B Virus in Vitro

1. The farst teachang Hospital ,Beijing

Abstract: uinolones were studied for their ability to inhibit hepatitis B virus DNA replicatiun,HBsAg andHBeAg exporssion in a HBV-transfeeted eell line(2.2.15 cell).The survival cell rate was determinedby MTT assay.The result shows that ID50(the drug concentration that inhibits HBsAg or HBeAg secretion by 50%) were 110g/ml and 199g/ml with Pipemidic Acid,64g/ml and 111g/ml with Norfloxacin,93g/ml and 210g/ml with Ciprofloxacin,105g/ml and 217g/ml with Ofloxacin.CD_50(the drug concentration that reduces cell growth by 50%) were 219g/ml,90g/ml,181g/ml,160g/ml,reepectively.In the range of drug concentration selected,they partially or completelybleeked the production of HBV particles and its replicative interniediates,especially the seneration of suporcoiled viral DNA((sc DNA).