Detection of Human Cytomegalovirus in Neonatal Hepatitis by Nested PCR

CHAN Xiang-Nian; CHEN Jin-Dong; YAN Meng; LU Xiao; QIU Ding-Gong

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April 1994

CHAN Xiang-Nian, CHEN Jin-Dong, YAN Meng, LU Xiao and QIU Ding-Gong. Detection of Human Cytomegalovirus in Neonatal Hepatitis by Nested PCR[J]. Virologica Sinica, 1994, 9(2): 125-129.

Citation: CHAN Xiang-Nian, CHEN Jin-Dong, YAN Meng, LU Xiao, QIU Ding-Gong. Detection of Human Cytomegalovirus in Neonatal Hepatitis by Nested PCR .VIROLOGICA SINICA, 1994, 9(2) : 125-129.

套式PCR检测婴幼儿巨细胞病毒感染

1.
南京铁道医学院生物教研室，南京210009

摘要

本文采用套式ＰＣＲ（Ｎｅｓｔｅｄｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ）技术对６１例婴儿肝炎综合征（Ｎｅｏｎａｔａｌｈｅｐａｔｉｔｉｓ）患者进行了ＨＣＭＶ（Ｈｕｍａｎｃｙｔｏｍｅｇａｌｏｖｉｒｕｓ）检测，结果表明，当只用一对引物进行ＰＣＲ时，检出３３例患者为ＨＣＭＶ阳性，阳性检出率为５４．１％；而采用套式ＰＣＲ后，有１７例为ＨＣＭＶ阳性，阳性检出率提高到７７．０％。可见套式ＰＣＲ方法的阳性检出率明显高于单一ＰＣＲ，并远高于目前使用的其它方法。同时也说明ＨＣＭＶ是导致肝炎综合征的主要病原体之一。此外，灵敏性和特异性试验表明，套式ＰＣＲ技术的敏感度高、特异性强、简单快速，在临床检测和诊断中具有很大的潜力。
- 套式多聚酶链反应
- , 婴儿肝炎综合征
- , 巨细胞病毒

Detection of Human Cytomegalovirus in Neonatal Hepatitis by Nested PCR

1.
Department of biology ,Nanjing Railary Medical College ，Nanjing 210009

Abstract

ixty-one children patients with neonatal hepatitis were investigated for human cytomesalovirus(HCMV)by single PCR and nested double PCR.of the 61 patients,33(54.1%)were positive forHCMV by single PCR,whereas 47(77.0%)were positive by nested double PCR.This showed that nest-ed double PCR is more sensitive than single PCR,and also revealed that HCMV can be one of the important agents for neonatal hepotitis.In addition,its advantage of high sensitivity and specificty com-bined with more rapid detection suggests that this precedure can be a potential diagnostic and epidemio-logical tool.
- Nested double PCR
- , Neonatal hepetitis
- , Human cytomegalovirus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Human Cytomegalovirus in Neonatal Hepatitis by Nested PCR

1. Department of biology ,Nanjing Railary Medical College ，Nanjing 210009

Abstract: ixty-one children patients with neonatal hepatitis were investigated for human cytomesalovirus(HCMV)by single PCR and nested double PCR.of the 61 patients,33(54.1%)were positive forHCMV by single PCR,whereas 47(77.0%)were positive by nested double PCR.This showed that nest-ed double PCR is more sensitive than single PCR,and also revealed that HCMV can be one of the important agents for neonatal hepotitis.In addition,its advantage of high sensitivity and specificty com-bined with more rapid detection suggests that this precedure can be a potential diagnostic and epidemio-logical tool.