Detection of Potato Leafroll Virus by Nucleic Acid Spot Hybridization with 32P Labelled cDNA Probe

HU Hui-Beng; HA Shi-A-Gu-La; ZHANG He-Ling

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February 1995

HU Hui-Beng, HA Shi-A-Gu-La and ZHANG He-Ling. Detection of Potato Leafroll Virus by Nucleic Acid Spot Hybridization with 32P Labelled cDNA Probe[J]. Virologica Sinica, 1995, 10(1).

Citation: HU Hui-Beng, HA Shi-A-Gu-La, ZHANG He-Ling. Detection of Potato Leafroll Virus by Nucleic Acid Spot Hybridization with 32P Labelled cDNA Probe .VIROLOGICA SINICA, 1995, 10(1) : 75.

用32P标记eDNA探针进行核酸斑点杂交检测马铃薯卷叶病毒(PLRV)

1.
内蒙古大学生物系

摘要

利用克隆的马铃薯卷叶病毒（ＰＬＲＶ）外壳蛋白（ＣＰ）基因测ｃＤＮＡ，用切口平移法制成￣（３２）Ｐ标记探针，通过核酸斑点杂交，对马铃薯卷叶病毒ＲＮＡ，提纯的马铃薯卷叶病毒和感染ＰＬＲＶ的马铃薯茎、叶、块茎及带毒桃蚜进行了检测。结果表明：ＰＬＲＶ－ＣＰ基因的ｃＤＮＡ探针特异性强，灵敏度高。可测出提纯的ＰＬＲＶ－ＲＮＡ的最低量为４０ｐｇ，感病马铃薯植株茎叶提取液的最高稀度为１：７５。探针只与ＰＬＲＶ及其ＲＮＡ反应，而不与ＰＶＹ、ＰＶＳ等病毒及其ＲＮＡ反应。从而为马铃薯卷叶病毒的有效诊断，建立了一项比血清学更为可靠、灵敏、特异性强，同时又适于大量样品检测的分子生物学方法。
- 马铃薯卷叶病毒（32）P中标记cDNA探针核酸斑点杂交诊断

Detection of Potato Leafroll Virus by Nucleic Acid Spot Hybridization with 32P Labelled cDNA Probe

1.
Dep. of Biology,Inner Mongonia University

Abstract

The cDNA of potato leafroll virus coat protein gene and cDNA by random primer were used as probe labeled with 32p by nick translation.
- Potato Lea froll Virus