Morphology study on Fixed Rabies Virus(Beijing Strain)in BHK_(21) Cells by Electron Microscopy

LI De-Zhong; WU Xiao-Hua; JIE Bing

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April 1995

LI De-Zhong, WU Xiao-Hua and JIE Bing. Morphology study on Fixed Rabies Virus(Beijing Strain)in BHK_(21) Cells by Electron Microscopy[J]. Virologica Sinica, 1995, 10(2).

Citation: LI De-Zhong, WU Xiao-Hua, JIE Bing. Morphology study on Fixed Rabies Virus(Beijing Strain)in BHK_(21) Cells by Electron Microscopy .VIROLOGICA SINICA, 1995, 10(2) : 125.

狂犬病毒“北京株”在地鼠肾传代细胞中增殖的电镜观察

1.
广州军区武汉总医院电镜室
2.
广州军区武汉总医院检验科

摘要

用透射电子显微镜观察术，对狂犬病固定毒“北京株”在地鼠肾传代细胞（８ＨＫ＿（２１））中增殖的形态学变化进行了研究。在受感染的细胞中有大量子弹状和长杆状的病毒颗粒。大多数病毒在扩张的内质网膜上以出芽的方式发生，凸向内质网腔内并逐渐发育成子弹状和杆状的成熟病毒。含病毒的内质网周围常伴有颗粒状或丝状均质区域，少数病毒在细胞膜上芽生。细胞间隙中亦可见病毒。还见病毒在细胞核膜内、外层上芽生，核周间隙中有许多病毒，有的与核边缘染色质密切接触并使其缺损。细胞核有的肿胀，有的浓缩，有的核内有较多的染色质间颗粒和周颗粒，但均未见病毒颗粒。表明该病毒的发生发育对细胞的膜性系统有密切依赖性，同时该病毒对宿主细胞有破坏作用。
- 狂犬病毒
- , BHK（21）细胞
- , 电镜观察

Morphology study on Fixed Rabies Virus(Beijing Strain)in BHK_(21) Cells by Electron Microscopy

1.
Wuhan General Hospital
2.
Guangzhou Military Region

Abstract

The BHK_21 cells infected with Beijing strain of fixed rabies virus were studied by electron mlcroscopy,The rod-like,bullet,round and oval viruses within the cytoplasm,mostly Iocated in the var-ious cisterna of rough and smooth endoplasmic reticulum and usually associated with homogeneous material masses.The virus may bud from their membranes towards the cisterna,protrude from the cellmembranes or dissociate in extracellular spaces.It was also found that viral formation and growth alsooccured in the nucl eus...
- Rabies virus
- , BHK21 cells
- , Electron microscopy

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Morphology study on Fixed Rabies Virus(Beijing Strain)in BHK_(21) Cells by Electron Microscopy

1. Wuhan General Hospital
2. Guangzhou Military Region

Abstract: The BHK_21 cells infected with Beijing strain of fixed rabies virus were studied by electron mlcroscopy,The rod-like,bullet,round and oval viruses within the cytoplasm,mostly Iocated in the var-ious cisterna of rough and smooth endoplasmic reticulum and usually associated with homogeneous material masses.The virus may bud from their membranes towards the cisterna,protrude from the cellmembranes or dissociate in extracellular spaces.It was also found that viral formation and growth alsooccured in the nucl eus...