Morphology and Biochemical Characteristics of the Nuclear Polyhedrosis Virus of Lymantria xylina Swinhoe

PENG Gui-Hong; ZHANG Li-Ren; CHEN Li-Hua; CHEN Yao-Shun

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April 1995

PENG Gui-Hong, ZHANG Li-Ren, CHEN Li-Hua and CHEN Yao-Shun. Morphology and Biochemical Characteristics of the Nuclear Polyhedrosis Virus of Lymantria xylina Swinhoe[J]. Virologica Sinica, 1995, 10(2).

Citation: PENG Gui-Hong, ZHANG Li-Ren, CHEN Li-Hua, CHEN Yao-Shun. Morphology and Biochemical Characteristics of the Nuclear Polyhedrosis Virus of Lymantria xylina Swinhoe .VIROLOGICA SINICA, 1995, 10(2) : 147.

木毒蛾核型多角体病毒形态结构及理化性质的研究

1.
中国科学院武汉病毒研究所
2.
福建省宁德地区林业局

摘要

木毒蛾核型多角体病毒属昆虫杆状病毒科，核型多角体病毒属，多粒包埋型。在扫描电镜下，多角体呈不规则多面体，大小下一，平均直径为１．４μｍ，病毒粒子杆状，大小约为３９４×５６ｎｍ，经ＳＤＭ－ＰＡＧＥ分析，病毒多角体蛋白分子量为３０．５ｋＤ，病毒粒子结构蛋白由１９条多肽组成，分子量范围在８８－１７ｋＤ之间。多角体富含Ｇｌｕ、Ｖａｌ、Ｌｅｕ、而Ｃｙｓ、Ｍｅｔ、和Ｈｉｓ含量较少。其酸碱氨基酸之比为１．２６。病毒核酸为一环状ＤＮＡ，长度约为３８μｍ，经ＨｉｎｄⅢ、ＰｓｔⅠ、ＥｃｏＲⅠ和ＢｇｌⅠ单酶切以及ＢａｍＨⅠ＋ＨｉｎｄⅢ、ＢａｍＨⅠ＋ＰｓｔⅠ、ＢａｍＨⅠ＋ＢｇＩⅠ和Ｘｈｏｌ＋ＥｃｏＲⅠ双酶切分析，ＤＮＡ总分子量约为７１．６×１０￣６Ｄａｌｔｏｎｓ。
- 木毒蛾
- , 核型多角体病毒
- , 形态结构
- , 生化特性

Morphology and Biochemical Characteristics of the Nuclear Polyhedrosis Virus of Lymantria xylina Swinhoe

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

ymantria xylina Swinhoe nuclear polyhedrcais virus (LxNPV)is a kind of multi-embeded baculovirus. Scanning electron microscope(SEM)showed that the LxNPV inclusion bodies were irregularpolyhedra with different sizes.The average diameter of the polyhedra was 1.40μm.A lot of rod-shapedvirions which had a mean size of 394×56nm were released from polyhedra when they were decomposed by alkali. The virus genome was a circular DNA macromolecule with lensth of 38.0μm.SDS-PAGE showed that polyhetlrin had only one ma...
- Lymantria xylina Swinhoe
- , Nuclear polyhedrosis virus
- , Fine structure
- , Bieehemical characteristics

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Morphology and Biochemical Characteristics of the Nuclear Polyhedrosis Virus of Lymantria xylina Swinhoe

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: ymantria xylina Swinhoe nuclear polyhedrcais virus (LxNPV)is a kind of multi-embeded baculovirus. Scanning electron microscope(SEM)showed that the LxNPV inclusion bodies were irregularpolyhedra with different sizes.The average diameter of the polyhedra was 1.40μm.A lot of rod-shapedvirions which had a mean size of 394×56nm were released from polyhedra when they were decomposed by alkali. The virus genome was a circular DNA macromolecule with lensth of 38.0μm.SDS-PAGE showed that polyhetlrin had only one ma...