Rabbit haemorrhagic disease virus:Isolation of messenger RNA and identification of its activity

ZUO Li; DONG Xi-Chang; SUN Xiao-Juan

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April 1995

ZUO Li, DONG Xi-Chang and SUN Xiao-Juan. Rabbit haemorrhagic disease virus:Isolation of messenger RNA and identification of its activity[J]. Virologica Sinica, 1995, 10(2).

Citation: ZUO Li, DONG Xi-Chang, SUN Xiao-Juan. Rabbit haemorrhagic disease virus:Isolation of messenger RNA and identification of its activity .VIROLOGICA SINICA, 1995, 10(2) : 169.

黄芪A_6组分与无环鸟苷联合对Ⅰ型单纯疱疹病毒感染小鼠的治疗作用

1.
贵阳医学院微牛物学教研室，海南医学院微生物学教研室

摘要

黄芪Ａ＿６组分与无环鸟苷联合对Ⅰ型单纯疱疹病毒感染小鼠的治疗作用左丽，董熙昌，孙晓娟（贵阳医学院微牛物学教研室，贵阳５５０００４）关键词黄芪，无环鸟苷，药物协同作用，Ⅰ型中纯疱疹病毒，小鼠单纯疱疹病毒（ＨＳＶ）是人类最常见的病原之一，可引起多种疱疹性...
- 黄芪
- , 无环鸟苷
- , 药物协同作用
- , Ⅰ型中纯疱疹病毒
- , 小鼠

Rabbit haemorrhagic disease virus:Isolation of messenger RNA and identification of its activity

Abstract

poly(A)tRNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)tRNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with molecular weight of 64,59,54,51,29,24 and 20kD wereshown RHDV-specific.
- Rabbit haemorrhagic disease virus Messenger RNA Polysome immunoprecipitation in vit ro translation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Rabbit haemorrhagic disease virus:Isolation of messenger RNA and identification of its activity

Abstract: poly(A)tRNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)tRNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with molecular weight of 64,59,54,51,29,24 and 20kD wereshown RHDV-specific.