The Analysis of the Struetural Polypeptides of
Rabbit Hemorrhagic Disease Virus

ZHANG Quan-Shun; DU Nian-Xin; XU Wei-Yan

PDF
Cite
Share

facebook

twitter

google

linkedin

June 1995

ZHANG Quan-Shun, DU Nian-Xin and XU Wei-Yan. The Analysis of the Struetural Polypeptides ofRabbit Hemorrhagic Disease Virus[J]. Virologica Sinica, 1995, 10(3).

Citation: ZHANG Quan-Shun, DU Nian-Xin, XU Wei-Yan. The Analysis of the Struetural Polypeptides of Rabbit Hemorrhagic Disease Virus .VIROLOGICA SINICA, 1995, 10(3) : 258.

兔出血症病毒结构多肽分析

1.
南京农业大学动物医学院上海动植物检疫局

摘要

粗提病毒经Ｓｅｐｈａｒｏｓｅ４Ｂ层析后，获得纯化的兔出血症病毒（ＲＨＤＶ）。提纯病毒经过ＳＤＳ－ＰＡＧＥ经考马斯亮蓝染色显示Ａ、Ｂ、Ｃ、Ｄ、Ｅ、Ｆ和Ｇ７条多肽，凝胶扫描显示Ａ为ＲＨＤＶ主要结构多肽。用多抗和单抗作免疫转印分析，证实Ａ、Ｂ、Ｃ、Ｄ、Ｅ、和Ｇ为结构多肽，此６条结构多肽间的抗原关系十分密切。
- 兔出血症病毒 SDS PAGE 免疫转印

The Analysis of the Struetural Polypeptides of Rabbit Hemorrhagic Disease Virus

1.
Nanjing Agricultural University

Abstract

The RHDV virion comprised six sturctural polypeptides as analysed by sodium dodecylsulfate-polyacrylamide gel electrophoresis, including A(molecular weight 60.12 kD),B(58. 27kD),C(54.74kD),D(53. 01kD),E(51. 10kD) and G(25. 90kD).The major polypep-tide A(including polypeptide B constituted about 54.7% of the total viral proteins. polypep-tide C constituted 1 7.7%, polypeptide D and E were about 22.7%and polypeptide G wereabout 4.9%. By western blotting with rabbit antiserum,polypeptides A、B、C、D、E and Gwere detected. by using monoclonal antibodies AA8-1、CG4-1 and CH3-1,all the six polypep-tides could be recognized,indicating that these polypeptides shared extensive antigenic rela-tionship.
- Rabbit hemorrhagic disease virus SDS PAGE Immunoblotting

References
Proportional views

PDF

Article Metrics

Article views(3533) PDF downloads(719) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Analysis of the Struetural Polypeptides of Rabbit Hemorrhagic Disease Virus

1. Nanjing Agricultural University

Abstract: The RHDV virion comprised six sturctural polypeptides as analysed by sodium dodecylsulfate-polyacrylamide gel electrophoresis, including A(molecular weight 60.12 kD),B(58. 27kD),C(54.74kD),D(53. 01kD),E(51. 10kD) and G(25. 90kD).The major polypep-tide A(including polypeptide B constituted about 54.7% of the total viral proteins. polypep-tide C constituted 1 7.7%, polypeptide D and E were about 22.7%and polypeptide G wereabout 4.9%. By western blotting with rabbit antiserum,polypeptides A、B、C、D、E and Gwere detected. by using monoclonal antibodies AA8-1、CG4-1 and CH3-1,all the six polypep-tides could be recognized,indicating that these polypeptides shared extensive antigenic rela-tionship.