对戊型肝炎病毒（ＨＥＶ）编码蛋白序列进行了亲水性分析及二级结构预测，选择亲水性强、具有β－转角与β－折叠的区段，采用多肽固相合成法合成了ＨＥＶ基因组３个开读框架（ＯＲＦ１，ＯＲＦ２和ＯＲＦ３）中可能的抗原表位，以免疫学方法进行鉴定并选出了分别来自ＨＥＶ３个ＯＲＦ的、具有重要生物活性与应用前景的３段肽（ＥＨ１７４、ＥＨ２６５、ＥＨ３６２）。在此基础上，进行了抗ＨＥＶＥＬＩＳＡ新型检测试剂盒的实验室研究及临床试用。结果表明，所研究的戊肝抗体检测试剂盒特异性高、临床符合性好、具有可重复性，在戊肝辅助诊断及流行病学调查中具有重要意义。

Accordingto our computer analysis of hydrophobicity and prediction of secondary structuresfor the full-length putative proteins encoded by ORF 1(open reading frame-1),ORF2 and ORF3ofhepatitis E virus(HEV),we selected amino acid regions with tiydrophilicity,β-turn,and β-sheet,and synthesized seven peptities of possible epitope-containing regions of the polypeptide en-coded by all three ORFs of HEV genomic RNA by Merrifield s method of solid-phase,The syn-thetic peptides were screened and identified by solli-phase enzyme-linked immunosorbent assay(ELISA).Three of the peptides(EH174 from ORF1,EH286 from ORF2 and EH362 from ORF3)showed antigenic activity and possible application for the development of anti-HEV test k its(thepeptide-based EL ISA).The laboratory experunents and clinical trials indicated that the kits,us-ing a set of three synthetic HEV pepties as coating antigens,were of high specificity and exhibit-ed good reproducibility,Additionally,our results also demonstrated good agreement with clinical find i