Detection of pseudorabies virus by biotinylated DNA probe

WANG Qin; GUO Wan-Zhu; LIN Wen-Qi

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June 1996

WANG Qin, GUO Wan-Zhu and LIN Wen-Qi. Detection of pseudorabies virus by biotinylated DNA probe[J]. Virologica Sinica, 1996, 11(3).

Citation: WANG Qin, GUO Wan-Zhu, LIN Wen-Qi. Detection of pseudorabies virus by biotinylated DNA probe .VIROLOGICA SINICA, 1996, 11(3) : 284.

应用生物素标记DNA探针检测伪狂犬病病毒的研究

1.
四川农业大学动物科技学院四川畜牧兽医学院动医系

摘要

应用生物素标记ＤＮＡ探针检测伪狂犬病病毒的研究王琴，郭万柱，阴文奇（四川农业大学动物科技学院，四川雅安，６２５５０１４）关键词伪狂犬病毒，核酸，杂交，检测核酸杂交技术已广泛用于检测畜禽疱疹病毒和其它动物病毒，已报道ＰＲＶ－ＤＮＡ的探针方法有ＲＮＡ－Ｄ...
- 伪狂犬病毒核酸杂交检测

Detection of pseudorabies virus by biotinylated DNA probe

1.
Sichuan Agricultural University，Yaan Sichuan Province 625014

Abstract

dot blot hybridization method was developed to detect pseudorbies virus DNA in infectedcultured cells.Seven strains of pseudorabies virus were detected by hiotinylated pseudorabies virusFa DNA probe,and recomhinant plasmid pPB11DNA(BamHI-11 fragment of PRV Fa was clonedbetween the BamHI sites of plasmid pBR322)probe.PRV Fa DNA probe could detect 10 pg nu-cleic acid in the samples. The nucleic acid hybridization method is sensitive and specific for diag-nosing pseudorabies virus.
- Pseudorabies virus Nucleic acids Hybridization Detection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of pseudorabies virus by biotinylated DNA probe

1. Sichuan Agricultural University，Yaan Sichuan Province 625014

Abstract: dot blot hybridization method was developed to detect pseudorbies virus DNA in infectedcultured cells.Seven strains of pseudorabies virus were detected by hiotinylated pseudorabies virusFa DNA probe,and recomhinant plasmid pPB11DNA(BamHI-11 fragment of PRV Fa was clonedbetween the BamHI sites of plasmid pBR322)probe.PRV Fa DNA probe could detect 10 pg nu-cleic acid in the samples. The nucleic acid hybridization method is sensitive and specific for diag-nosing pseudorabies virus.