Amplification of the Intact Gene Coding Epstein-Barr Virus-Specific tIIym idine Kinase

CHEN Shang-Wu; CHEN Rui-Jun; HUANG Di; SHU Zhen-Yu; MA Jian-Quan

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June 1996

CHEN Shang-Wu, CHEN Rui-Jun, HUANG Di, SHU Zhen-Yu and MA Jian-Quan. Amplification of the Intact Gene Coding Epstein-Barr Virus-Specific tIIym idine Kinase[J]. Virologica Sinica, 1996, 11(3).

Citation: CHEN Shang-Wu, CHEN Rui-Jun, HUANG Di, SHU Zhen-Yu, MA Jian-Quan. Amplification of the Intact Gene Coding Epstein-Barr Virus-Specific tIIym idine Kinase .VIROLOGICA SINICA, 1996, 11(3) : 287.

EB病毒胸苷激酶基因的扩增

1.
中山医科大学生化教研室中山医科大学肿瘤研究所中山大学昆虫所

摘要

鼻咽癌(r 0pl1a舯g carcinoma，NPc)是我国华南及录雨豆碍瓣见的恶性肼；瘤．早期诊断是防治的关键措施之一。大量证据表明EB病毒(Epstein—Barr virus，EBV)与NPC密切相关【I】。EBV编码一个病毒特异的胸苷激酶(thymidirm kinase，TK)[21。研究发现，绝大多数NPC病人血清中具有EBVTK特异的抗体口]，提示研究EBVTK对于NPC的早期诊断、阐明其在NPC发生发展中的作用具有重要意义。本研究根据EBV DNA全序列资料】，自己设计一对PCR引物，用PCR方法扩增出完整的EBV TK基因，为EBV TK基因的检测及研究 EBV TK与NPC的关系奠定了基础。
- Epstein－Barr病毒胸苷激酶基因聚合酶链反应

Amplification of the Intact Gene Coding Epstein-Barr Virus-Specific tIIym idine Kinase

1.
Department of Biochemistry．Sun-Yet University of Medical Sciences，Guangzhou 510089Cancer Research InStitute，Sun-sen University of Medical＆iences．Guangzhou 510060

Abstract

Based o1"1 Epstein—Barr virus(EBV)DNA (B95—8)genome sequenc3e data and the strut—ture BXLFlopen readingframe codingforEBV ~hymidinekinase(TK)，a pairofPCR pr／mers was designed and svnthesized Template DNA was extracted rapidly from B95—8 calIs harboring EBV bv using guanidinium isothioeyanate(GuSCN)and silica∞urse(cs)．A 1843bp A frag—ment containing intact EBV TK gene was amplified by polymerase chain reaction．The PCR prod—uct was verified by cleaving with NcoI
- Epstein—Barr virus。Thymidine kiriase．Gene

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Amplification of the Intact Gene Coding Epstein-Barr Virus-Specific tIIym idine Kinase

1. Department of Biochemistry．Sun-Yet University of Medical Sciences，Guangzhou 510089Cancer Research InStitute，Sun-sen University of Medical＆iences．Guangzhou 510060

Abstract: Based o1"1 Epstein—Barr virus(EBV)DNA (B95—8)genome sequenc3e data and the strut—ture BXLFlopen readingframe codingforEBV ~hymidinekinase(TK)，a pairofPCR pr／mers was designed and svnthesized Template DNA was extracted rapidly from B95—8 calIs harboring EBV bv using guanidinium isothioeyanate(GuSCN)and silica∞urse(cs)．A 1843bp A frag—ment containing intact EBV TK gene was amplified by polymerase chain reaction．The PCR prod—uct was verified by cleaving with NcoI